{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:40:03Z","timestamp":1697589603089},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,5,9]],"date-time":"2002-05-09T00:00:00Z","timestamp":1020902400000},"content-version":"vor","delay-in-days":128,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The lining of the trachea consists of a pseudostratified, mucociliary epithelium that under a variety of conditions, such as vitamin A deficiency, toxic and mechanical injury, becomes a stratified squamous epithelium. Several in vitro cell culture models have been established to study the process of differentiation of airway epithelium. Such studies have indicated that mucosecretory differentiation of tracheal epithelial cells can be modulated by substratum. This study was undertaken to understand molecular mechanisms of squamous differentiation in tracheal epithelia. Primary cultured tracheal cells grown on uncoated filters were differentiated to single layer of squamous cells, whereas cells were grown as stratified columanar cells on collagen\u2010I coated filters. The responses to secretagogues were altered according to culture conditions. DD\u2010PCR revealed that FAK and a WD protein expression was increased in squamous tracheal epithelia. Expression of a WD protein was changed by the treatment of retinoic acid in various epithelial cells. These results indicated that squamous differentiation of tracheal cells changes the expression of a variety of genes, and that the experimental model for this study can be employed to study molecular mechanisms of squamous differentiation in airway epithelial cells. J. Cell. Biochem. 86: 194\u2013201, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10201","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:21:52Z","timestamp":1033906912000},"page":"194-201","source":"Crossref","is-referenced-by-count":2,"title":["Differential expression of a WD protein during squamous differentiation of tracheal epithelial cells"],"prefix":"10.1002","volume":"86","author":[{"given":"Y.S.","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.M.","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.J.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.S.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12313396"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00483-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1993.tb01602.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/9.3.264"},{"key":"e_1_2_7_6_1","first-page":"170","article-title":"Establishment and characterization of a continuous human basal cell carcinoma cell line from facial skin (I) cytological behavior of early passage","volume":"10","author":"Chiang LC","year":"1994","journal-title":"Kaohsiung J Med Sci"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1091\/mbc.1.11.791","article-title":"TGF\u2010beta and retinoic acid: regulators of growth and modifiers of differentiation in human epidermal cells","volume":"11","author":"Choi Y","year":"1990","journal-title":"Cell Regul"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287399209531613"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/15.3.503"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340130105"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12365200"},{"key":"e_1_2_7_12_1","first-page":"333","article-title":"Extracellular matrix regulation of cell\u2010cell communication and tissue\u2010specific gene expression in primary liver cultures","volume":"226","author":"Fujita M","year":"1986","journal-title":"Prog Clin Biol Res"},{"key":"e_1_2_7_13_1","first-page":"706","article-title":"Adhesion systems in normal breast and in invasive breast carcinoma","volume":"146","author":"Glukhova M","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(93)90088-8"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2613.1996.968106.x"},{"key":"e_1_2_7_16_1","first-page":"5","article-title":"Structure and function of mucus\u2010secreting cells of cat and goose airway epithelium","volume":"54","author":"Jeffery PK","year":"1978","journal-title":"Ciba Found Symp"},{"key":"e_1_2_7_17_1","first-page":"654","article-title":"Retinoic acid and substratum regulate the differentiation of rabbit tracheal epithelial cells into squamous and secretory phenotype. Morphological and biochemical characterization","volume":"56","author":"Jetten AM","year":"1987","journal-title":"Lab Invest"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90264-4"},{"key":"e_1_2_7_19_1","first-page":"93","article-title":"Control of squamous differentiation in tracheobronchial and epidermal epithelial cells: role of retinoids","volume":"13","author":"Jetten AM","year":"1992","journal-title":"J Natl Cancer Inst Monogr"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3468-6_12"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(00)00985-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201186"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02630999"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.3.960"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/371297a0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-2993(11)80468-9"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61347-9"},{"key":"e_1_2_7_28_1","first-page":"2752","article-title":"Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors","volume":"55","author":"Owens LV","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1289\/ehp.8980229","article-title":"Effect of substratum and retinoids upon the mucosecretory differentiation of airway epithelial cells in vitro","volume":"80","author":"Rearick JI","year":"1989","journal-title":"Environ Health Perspect"},{"key":"e_1_2_7_30_1","first-page":"601","article-title":"Modulation of cell spreading and migration by pp125FAK phosphorylation","volume":"147","author":"Sankar S","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_7_31_1","first-page":"73","article-title":"Ciliary function in mucus transport","volume":"116","author":"Sleigh MA","year":"1981","journal-title":"Am Rev Resp Dis"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01384-5"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.15.4985-4995.2001"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.5.1383"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.3.1416"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80751-2"},{"key":"e_1_2_7_37_1","first-page":"413","article-title":"Attenuation of the expression of the focal adhesion kinase induces apoptosis in tumor cells","volume":"7","author":"Xu LH","year":"1996","journal-title":"Cell Growth Differ"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.16.6.9191474"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.10201","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.10201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:20:48Z","timestamp":1697588448000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.10201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1002\/jcb.10201"],"URL":"https:\/\/doi.org\/10.1002\/jcb.10201","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]}}}