{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:29:50Z","timestamp":1790872190102,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,11,11]],"date-time":"2002-11-11T00:00:00Z","timestamp":1036972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gene silencing via promoter CpG island hypermethylation offers tumor cells growth advantages. This epigenetic event is pharmacologically reversible, and uncovering a unique set of methylation\u2010silenced genes in tumor cells can bring a new avenue to cancer treatment. However, high\u2010throughput tools capable of surveying the methylation status of multiple gene promoters are needed for this discovery process. Herein we describe an oligonucleotide\u2010based microarray technique that is both versatile and sensitive in revealing hypermethylation in defined regions of the genome. DNA samples are bisulfite\u2010treated and PCR\u2010amplified to distinguish CpG dinucleotides that are methylated from those that are not. Fluorescently labeled PCR products are hybridized to arrayed oligonucleotides that can discriminate between methylated and unmethylated alleles in regions of interest. Using this technique, two clinical subtypes of non\u2010Hodgkin's lymphomas, mantle cell lymphoma, and grades I\/II follicular lymphoma, were further separated based on the differential methylation profiles of several gene promoters. Work is underway in our laboratory to extend the interrogation power of this microarray system in multiple candidate genes. This novel tool, therefore, holds promise to monitor the outcome of various epigenetic therapies on cancer patients. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10313","type":"journal-article","created":{"date-parts":[[2002,12,3]],"date-time":"2002-12-03T13:51:53Z","timestamp":1038923513000},"page":"138-143","source":"Crossref","is-referenced-by-count":85,"title":["Oligonucleotide\u2010based microarray for DNA methylation analysis: Principles and applications"],"prefix":"10.1002","volume":"88","author":[{"given":"Huidong","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Maier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inko","family":"Nimmrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pearlly S.","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles W.","family":"Caldwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Olek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim Hui\u2010Ming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.5.e21"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000501"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90015-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/321209a0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.947102"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.15.2990"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00785-7"},{"key":"e_1_2_7_9_1","first-page":"3225","article-title":"A gene hypermethylation profile of human cancer","volume":"61","author":"Esteller M","year":"2001","journal-title":"Cancer 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progression\u2010free survival in patients and identifies candidate epigenetic markers","volume":"8","author":"Wei SH","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_23_1","first-page":"17","article-title":"The potential prognostic, predictive, and therapeutic values of DNA methylation in cancer","volume":"8","author":"Widschwendter M","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_24_1","first-page":"1432","article-title":"CpG island arrays: An application toward deciphering epgienetic signatures of breast cancer","volume":"6","author":"Yan P","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_25_1","first-page":"8375","article-title":"Dissecting complex epigenetic alterations in breast cancer using CpG island microarrays","volume":"61","author":"Yan PS","year":"2001","journal-title":"Cancer Res"}],"container-title":["Journal of Cellular 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