{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:38:58Z","timestamp":1791020338829,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,10,31]],"date-time":"2002-10-31T00:00:00Z","timestamp":1036022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is now well established that supraphysiological doses of 1\u03b1,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> [1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>] stimulate bone resorption. Recent studies have established that osteoblasts\/stromal cells express receptor activator of NF\u2010\u03baB ligand (RANKL) in response to several bone\u2010resorbing factors including 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> to support osteoclast differentiation from their precursors. Osteoclast precursors which express receptor activator of NF\u2010\u03baB (RANK) recognize RANKL through cell\u2010to\u2010cell interaction with osteoblasts\/stromal cells, and differentiate into osteoclasts in the presence of macrophage\u2010colony stimulating factor (M\u2010CSF). Osteoprotegerin (OPG) acts as a decoy receptor for RANKL. We also found that daily oral administration of 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> for 14 days to normocalcemic thyroparathyroidectomized (TPTX) rats constantly infused with parathyroid hormone (PTH) inhibited the PTH\u2010induced expression of RANKL and cathepsin K mRNA in bone. The inhibitory effect of 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> on the PTH\u2010induced expression of RANKL mRNA occurred only with physiological doses of the vitamin. Supraphysiological doses of 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> increased serum Ca and expression of RANKL in vivo in the presence of PTH. These results suggest that the bone\u2010resorbing activity of vitamin D does not occur at physiological dose levels in vivo. A certain range of physiological doses of 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> rather suppress the PTH\u2010induced bone resorption in vivo, supporting the concept that 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> or its derivatives are useful for the treatment of various metabolic bone diseases such as osteoporosis and secondary hyperparathyroidism. J. Cell. Biochem. 88: 259\u2013266, 2003. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10331","type":"journal-article","created":{"date-parts":[[2003,1,9]],"date-time":"2003-01-09T21:06:13Z","timestamp":1042146373000},"page":"259-266","source":"Crossref","is-referenced-by-count":190,"title":["Vitamin D and bone"],"prefix":"10.1002","volume":"88","author":[{"given":"Tatsuo","family":"Suda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Ueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyuki","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimasa","family":"Shinki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,1,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.11.7110"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/36593"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1952.tb00904.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.3.3280376"},{"key":"e_1_2_7_6_1","first-page":"695","volume-title":"Vitamin D","author":"Easel R","year":"1998"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.1.175"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9831"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1210\/endo.141.1.7229","article-title":"Dynamic regulation of RGS2 in bone: Potential new insights into parathyroid hormone signaling mechanisms","volume":"141","author":"Miles RR","year":"2000","journal-title":"Endocrinology"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9788"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4023.768"},{"key":"e_1_2_7_13_1","first-page":"1169","volume-title":"Osteoporosis","author":"Reid IR","year":"1998"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.6.2581"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900704"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80209-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/100.9.1049"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-13-1-66"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(95)00185-G"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.20.3.0367"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-5-2600"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.2.6673"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6603"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.18.7260"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.40.25190"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.3.5837"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3597"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0897-391"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.10331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.10331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T18:58:20Z","timestamp":1697482700000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.10331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1,6]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,2]]}},"alternative-id":["10.1002\/jcb.10331"],"URL":"https:\/\/doi.org\/10.1002\/jcb.10331","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1,6]]}}}