{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:05:09Z","timestamp":1778695509367,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,5,19]],"date-time":"2003-05-19T00:00:00Z","timestamp":1053302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the relevance of gelatinase\u2010B (matrix metalloproteinase 9, MMP\u20109) in gouty arthritis (GA), we tested the occurrence of MMP\u20109 in GA patients and cell culture system. Gelatinolytic activity in the synovial fluid (SF) of patients with different kinds of arthritis was assessed by gelatin zymography. A predominant 92\u2010kDa MMP\u20109 gelatinolytic activity was evident in rheumatoid arthritis (RA) and GA samples, but no activity was observed in osteoarthritis (OA) samples. Among the 53 SF samples (9 RA, 24 GA, and 20 OA) analyzed for MMP\u20109 and tissue inhibitor of metalloproteinase (TIMP\u20101) antigen levels by ELISA, MMP\u20109 antigen levels were elevated tenfold in GA SF compared with OA SF. In addition, GA synovial tissue extracts revealed elevated levels of MMP\u20109 expression as compared to OA tissue extracts by Western blot and RT\u2010PCR analysis. Immunohistochemical studies demonstrated that MMP\u20109 immunoreactivity was more intense in GA than in OA synovial tissues. Furthermore, macrophages activation by gouty crystals in vitro was examined. Crystals stimulated MMP\u20109 gene expression in macrophage cell line and such stimulation was suppressed by PD98059. These findings suggest that the abnormal production of MMP\u20109 by macrophages is a reflection of the pathological conditions in joints of patients with GA, and that the activation of MMP\u20109 in the joint is known to play an important role in joint disease. J. Cell. Biochem. 89: 791\u2013799, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10530","type":"journal-article","created":{"date-parts":[[2003,7,8]],"date-time":"2003-07-08T10:06:08Z","timestamp":1057658768000},"page":"791-799","source":"Crossref","is-referenced-by-count":26,"title":["Expression of matrix metalloproteinase\u20109 (gelatinase B) in gouty arthritis and stimulation of MMP\u20109 by urate crystals in macrophages"],"prefix":"10.1002","volume":"89","author":[{"given":"Ming\u2010Shium","family":"Hsieh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Chiu","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Der\u2010Tsay","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiann","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Chih","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsu\u2010Yi","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joung\u2010Liang","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010Huei","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,6,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200105000-00015"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780390919"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290816"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1950159"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400525"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.4049\/jimmunol.138.10.3213","article-title":"Interleukin 1 (IL1) as a mediator of crystal arthritis: Stimulation of T cell and synovial fibroblast mitogenesis by urate crystal\u2010induced IL\u20101","volume":"138","author":"di Giovine FS","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115142"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1995.197"},{"key":"e_1_2_5_11_1","first-page":"1309","article-title":"Stromelysin and tissue inhibitor of metalloproteinase gene expression in rheumatoid arthritis synovium","volume":"140","author":"Firestein GS","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"7910","DOI":"10.1523\/JNEUROSCI.16-24-07910.1996","article-title":"Matrix metalloproteinase\u20109 (MMP\u20109) is synthesized in neurons of the human hippocampus and is capable of degrading the amyloid\u2010beta peptide (1\u201340)","volume":"16","author":"Fujimoto N","year":"1996","journal-title":"J Neurosci"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780321114"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.6.2019"},{"key":"e_1_2_5_15_1","first-page":"593","article-title":"Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis","volume":"19","author":"Hirose T","year":"1992","journal-title":"J Rheumatol"},{"key":"e_1_2_5_16_1","first-page":"79","article-title":"A one\u2010step sandwich enzyme immunoassay for inactive precursor and complexed forms of human matrix metalloproteinase 9 (92 kDa gelatinase\/type IV collagenase, gelatinase B) using monoclonal antibodies","volume":"23","author":"Hosokawa N","year":"1994","journal-title":"Clin Chim Acta"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(98)80122-1"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.58.11.691"},{"key":"e_1_2_5_19_1","first-page":"385","article-title":"Activated gelatinase\u2010B (MMP\u20109) and urokinase\u2010type plasminogen activator in synovial fluids of patients with arthritis. 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