{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:45:04Z","timestamp":1791096304509,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,3,31]],"date-time":"2003-03-31T00:00:00Z","timestamp":1049068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Activated T cells (Act T) produce multiple cytokines that affect osteoblast function as well as osteoclastogenesis. One of these cytokines, IL\u201013, is a multifunctional cytokine elaborated by Act T that regulates vascular cellular adhesion molecule (VCAM)\u20101 expression in endothelial cells. VCAM\u20101 has also been implicated in osteoclast formation by myeloma cells. We therefore studied whether IL\u201013 regulates VCAM\u20101 in human osteoblastic cells since these cells express RANKL, the major osteoclastogenic factor and osteoclast precursors are found adjacent to osteoblasts. Human T cells were activated in the absence or presence of Cyclosporin A (CsA), an inhibitor of the production of most activated T cell cytokines. Conditioned media were assayed for IL\u201013 by ELISA. Act T produced IL\u201013 and, unlike other T cell cytokines, this was elevated 3\u2010fold by CsA. Exposure of human osteoblasts (hOB) to doses of recombinant human IL\u201013 (rhIL\u201013, 0\u201310 ng\/ml) resulted in an increase of VCAM\u20101 mRNA (up to 5\u2010fold) within 4 h with a maximum stimulation at 1 ng\/ml. CsA had no effect on basal hOB VCAM\u20101 mRNA expression. Examination of VCAM\u20101 on the cell surface of hOB, by immunocytochemistry, revealed increasing levels of surface expression of the protein within 16 h after stimulation with doses of rhIL\u201013 (0.1\u201310 ng\/ml) which were reflective of the mRNAs. IL\u20106 production was also stimulated in a dose dependent manner with a maximum of 2.5\u2010fold with 1 ng\/ml rhIL\u201013 within 16 h. Since both VCAM\u20101 and IL\u20106 showed similar responses to IL\u201013, IL\u20106 was examined for its ability to induce VCAM\u20101. Immunocytochemistry demonstrated no effect of IL\u20106 on VCAM\u20101 expression. These data demonstrate that during pathological processes associated with T cell activation, such as rheumatoid arthritis or possibly post\u2010menopausal osteoporosis, T cells may play a pivotal role in osteoclast precursor adhesion to osteoblasts as a first step prior to RANKL signaling. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10531","type":"journal-article","created":{"date-parts":[[2003,4,16]],"date-time":"2003-04-16T10:45:31Z","timestamp":1050489931000},"page":"213-219","source":"Crossref","is-referenced-by-count":10,"title":["IL\u201013 regulates vascular cell adhesion molecule\u20101 expression in human osteoblasts"],"prefix":"10.1002","volume":"89","author":[{"given":"Leonard","family":"Rifas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Li","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"799","DOI":"10.4049\/jimmunol.154.2.799","article-title":"IL\u201013 selectively induces vascular cell adhesion molecule\u20101 expression in human endothelial cells","volume":"154","author":"Bochner BS","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650101018"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.6.8641176"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200105)44:5<985::AID-ANR176>3.0.CO;2-P"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.1.8275945"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90661-W"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01495-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.83.9.3285"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00449-X"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1621100"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.11.4226"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.5.1953"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90775-7"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950151106"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.7.1096"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.134.1.8275936"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.9.7649114"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10436"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650070502"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(01)00500-2"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1182\/blood.V84.6.1913.1913","article-title":"Regulation of endothelial and mesothelial cell function by interleukin\u201013: Selective induction of vascular cell adhesion molecule\u20101 and amplification of interleukin\u20106 production","volume":"84","author":"Sironi M","year":"1994","journal-title":"Blood"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650101006"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1996.00170.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.4049\/jimmunol.156.5.1818","article-title":"Human IL\u201013 production is negatively influenced by CD3 engagement. 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