{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T12:42:24Z","timestamp":1767703344937},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,6,25]],"date-time":"2003-06-25T00:00:00Z","timestamp":1056499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>For many types of cells, heat stress leads to an increase in intracellular free calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) that has been shown to trigger a wide variety of cellular responses. In T lymphocytes, for example, heat stress stimulates pathways that make them more susceptible to Fas\/CD95\u2010mediated apoptosis. Because of our interest in understanding more about the response of lymphocytes to various stressors, we used human peripheral and Jurkat T lymphocytes to investigate the effect of heat stress on calcium homeostasis. We found that peripheral and Jurkat T cells both exhibit cNOs activity but not iNOs activity. Heat stress increased NO production, which was inhibited by LNNA (a cNOs inhibitor) but not L\u2010NIL (an iNOs inhibitor). Heat stress increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in Jurkat T cells by decreasing the K<jats:sub>m<\/jats:sub> of the cell surface membrane Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger for extracellular Ca<jats:sup>2+<\/jats:sup>. Heating also increased cNOs phosphorylation at tyrosine residues. In cells incubated with LNNA, heat stress promoted an increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and a decrease in [Na<jats:sup>+<\/jats:sup>]<jats:sub>i<\/jats:sub> greater than in cells heated without LNNA, a larger decrease in K<jats:sub>m<\/jats:sub> of the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger for extracellular Ca<jats:sup>2+<\/jats:sup>, and decreased phosphorylation of cNOs. Our results suggest that cNOs plays an important regulatory role after heat stress. Heating appears to increase the phosphorylation of cNOs that is complexed with the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger to decrease its activity. This process is related to increased expression of Fas\/CD95 on the cell surface, which might explain the apoptotic diathesis of lymphocytes after heat stress. J. Cell. Biochem. 89: 1030\u20131043, 2003. Published 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10564","type":"journal-article","created":{"date-parts":[[2003,7,17]],"date-time":"2003-07-17T10:09:47Z","timestamp":1058436587000},"page":"1030-1043","source":"Crossref","is-referenced-by-count":16,"title":["Constitutive NO synthase regulates the Na<sup>+<\/sup>\/Ca<sup>2+<\/sup> exchanger in human T cells: Role of [Ca<sup>2+<\/sup>]<sub>i<\/sub> and tyrosine phosphorylation"],"prefix":"10.1002","volume":"89","author":[{"given":"Juliann G.","family":"Kiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"McClain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishal G.","family":"Warke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Krishnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George C.","family":"Tsokos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.17.9142"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3240971"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.9808"},{"key":"e_1_2_6_5_1","first-page":"144","article-title":"Increases in HSF1 translocation and synthesis in human epidermoid A\u2010431 cells: Role of protein kinase C and [Ca2+]i","volume":"44","author":"Ding XZ","year":"1996","journal-title":"J Investig Med"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2494(96)84924-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1096\/fasebj.12.10.773","article-title":"Expressional control of the constitutive isoforms of nitric oxide synthase (NOSI and NOSIII)","volume":"12","author":"Forstermann U","year":"1998","journal-title":"FASEB J"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/33934"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(99)00023-7"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.14.10453"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.9265"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003788200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199703000-00001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(91)90002-Y"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022836616452"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.6.C1561"},{"key":"e_1_2_6_17_1","first-page":"153","article-title":"N\u03c9\u2010nitro\u2010l\u2010arginine decreases resting cytosolic [Ca2+] and enhances heat stress\u2010induced increase in cytosolic [Ca2+] in human colon carcinoma T84 cells","volume":"42","author":"Kiang JG","year":"1999","journal-title":"Chin J Physiol"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(98)00028-X"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.1.C30"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"C104","DOI":"10.1152\/ajpcell.1994.267.1.C104","article-title":"HSP\u201072 synthesis is promoted by increase in [Ca2+]i or activation of G proteins but not pHi or cAMP","volume":"265","author":"Kiang JG","year":"1994","journal-title":"Am J Physiol"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1997.8364"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00138.2001"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.13.5159"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50223-X"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(05)80006-4"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"5668","DOI":"10.4049\/jimmunol.158.12.5668","article-title":"Overexpression of the heat shock protein 70 enhances the TCR\/CD3\u2010 and Fas\/Apo\/CD95\u2010mediated apoptotic cell death in Jurkat cells","volume":"158","author":"Liossis S\u2010NC","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107755200"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1523\/JNEUROSCI.17-07-02645.1997","article-title":"Nitric oxide facilitates long\u2010term potentiation, but not long\u2010term depression","volume":"17","author":"Malen PL","year":"1997","journal-title":"J Neurosci"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.749"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.4.1519"},{"key":"e_1_2_6_31_1","first-page":"109","article-title":"Nitric oxide: Physiology, pathophysiology, and pharmacology","volume":"43","author":"Moncada S","year":"1991","journal-title":"Pharmacol Rev"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101489200"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1242\/jeb.196.1.375","article-title":"Na+\/Ca2+ antiport in the mammalian heart","volume":"196","author":"Reeves JP","year":"1994","journal-title":"J Exp Biol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(96)70055-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(99)00038-6"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0497-336"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(99)00016-X"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199408000-00011"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.1.267"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/2940"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.45.28187"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6440"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111158598"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.10564","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.10564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T00:37:07Z","timestamp":1700267827000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.10564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,15]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1002\/jcb.10564"],"URL":"https:\/\/doi.org\/10.1002\/jcb.10564","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7,15]]}}}