{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:45:31Z","timestamp":1791143131295,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,9,17]],"date-time":"2003-09-17T00:00:00Z","timestamp":1063756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2003,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the differentiation of a variety of tissues, there is little information in regards to normal stromal cells. We investigated erbB2 expression in the murine fibroblast cell line Swiss 3T3L1, which naturally or hormonally induced undergoes adipocyte differentiation. We found that the Swiss 3T3\u2010L1 fibroblasts express erbB2, in addition to EGFR, and in a quantity comparable to or even greater than the breast cancer cell line T47D. Proliferating cells increased erbB2 and EGFR levels when reaching confluence up to 4\u2010 and 10\u2010fold, respectively. This expression showed a significant decrease when growth\u2010arrested cells were stimulated to differentiate with dexamethasone and isobutyl\u2010methylxanthine. Differentiated cells presented a decreased expression of both erbB2 and EGFR regardless of whether the cells were hormonally or spontaneously differentiated. EGF stimulation of serum\u2010starved cells increased erbB2 tyrosine phosphorylation and retarded erbB2 migration in SDS\u2013PAGE, suggesting receptor association and activation. Heregulin\u2010\u03b11 and \u2010\u03b21, two EGF related factors, had no effect on erbB2 or EGFR phosphorylation. Although 3T3\u2010L1 cells expressed heregulin, its specific receptors, erbB3 and erbB4, were not found. This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation. Since erbB2 and EGFR expression were downmodulated as differentiation progressed it is conceivable that a mechanism of switching from a mitogenic to a differentiating signaling pathway may be involved, through regulation of the expression of these growth factor receptors. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.10647","type":"journal-article","created":{"date-parts":[[2003,9,29]],"date-time":"2003-09-29T07:41:01Z","timestamp":1064821261000},"page":"561-572","source":"Crossref","is-referenced-by-count":17,"title":["ErbB2 and EGFR are downmodulated during the differentiation of 3T3\u2010L1 preadipocytes"],"prefix":"10.1002","volume":"90","author":[{"given":"Eleonora","family":"Pagano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan 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