{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T13:21:00Z","timestamp":1768396860908,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,3,15]],"date-time":"2001-03-15T00:00:00Z","timestamp":984614400000},"content-version":"vor","delay-in-days":73,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Complete understanding of the physiology and pathology of the cartilage is essential to establish treatments for a variety of cartilage disorders and defects such as rheumatoid arthritis, congenital malformations, and tumors of cartilage. Although synthetic materials have been used in many cases, they possess inherent problems including wear of the materials and low mechanical strength. Autograft has been considered very effective to overcome these problems. However, the limitation of the transplant volume is a major problem in autograft to be overcome. The costal cartilage is the most serious candidate for donor site transplantation, since it is the largest permanent hyaline cartilage in the body. To investigate the possibility using the costal cartilage as a transplant source, we have established and characterized three mouse chondrocyte cell lines (MCC\u20102, MCC\u20105, and MCC\u201035) derived from the costal cartilage of 8\u2010week\u2010old male SV40 large T\u2010antigen transgenic mice. At confluence, all the cell lines formed nodules that could be positively stained with alcian blue (pH 2.5). The size of nodules gradually increased during culturing time. After 2 and 6 weeks of culture, RT\u2010PCR analysis demonstrated that all three cell lines expressed mRNA from the cartilage\u2010specific genes for type II collagen, type XI collagen, aggrecan, and link protein. Furthermore, type X collagen expression was detected in MCC\u20105 and MCC\u201035 but not in MCC\u20102. Any phenotypic changes were not observed over 31 cell divisions. Immunocytochemistry showed further that MCC\u20102, MCC\u20105, and MCC\u201035 produced cartilage\u2010specific proteins type II collagen and type XI collagen, while in addition MCC\u20105 and MCC\u201035 produced type X collagen. Treatment with 1\u03b1, 25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> inhibited cell proliferation and differentiation of the three cell lines in a dose\u2010dependent manner. These phenotypic characteristics have been found consistent with chondrocyte cell lines established from cartilage tissues other than costal cartilage. In conclusion, costal cartilage shows phenotypic similarities to other cartilages, i.e., articular cartilage and embryonic limbs, suggesting that costal cartilage may be very useful as the donor transplantation site for the treatment of cartilage disorders. Furthermore, the cell lines established in this study are also beneficial in basic research of cartilage physiology and pathology. J. Cell. Biochem. 81: 571\u2013582, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.1075","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T23:03:55Z","timestamp":1031699035000},"page":"571-582","source":"Crossref","is-referenced-by-count":33,"title":["Establishment and characterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic 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