{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T13:49:51Z","timestamp":1747748991837},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,7,13]],"date-time":"2001-07-13T00:00:00Z","timestamp":994982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The role of regucalcin, which is a regulatory protein of calcium signaling, in the regulation of protein phosphatase activity in the nuclei of rat kidney cortex was investigated. Protein phosphatase activity towards phosphotyrosine, phosphoserine, and phosphothreonine was found in the nuclei. The enzyme activity towards three phosphoamino acids was significantly increased by the addition of calcium chloride (10\u201350 \u03bcM) in the enzyme reaction mixture. This increase was significantly inhibited by trifluoperazine (25 or 50 \u03bcM), an antagonist of calmodulin. The presence of regucalcin (50 or 100 nM) in the enzyme reaction mixture caused a significant decrease in protein phosphatase activity towards three phosphoamino acids. This effect was also seen in the presence of calcium (25 \u03bcM) and\/or calmodulin (5 \u03bcg\/ml). Protein phosphatase activity towards three phosphoamino acids was significantly increased in the presence of anti\u2010regucalcin monoclonal antibody (25 or 50 ng\/ml) in the enzyme reaction mixture. This effect was completely blocked by the addition of regucalcin (100 nM). The effect of antibody (25 ng\/ml) in increasing protein phosphatase activity towards phosphotyrosine was significantly inhibited by vanadate (10<jats:sup>\u22124<\/jats:sup> M). Also, the antibody's effect towards phosphoserine and phosphothreonine was significantly inhibited by cyclosporin A (10<jats:sup>\u22125<\/jats:sup> M). Endogenous regucalcin was found in the nuclei of rat kidney cortex using Western blot analysis. Nuclear regucalcin level was significantly reduced by the administration of saline (0.9% NaCl) for seven days in rats. Protein phosphatase activity towards three phosphoamino acids was significantly decreased by saline administration. The effect of anti\u2010regucalcin monoclonal antibody (25 ng\/ml) in increasing protein phosphatase activity towards three phosphoamino acids was weakened in the renal cortex nuclei of saline\u2010administrated rats. The present study demonstrates that endogenous regucalcin plays a suppressive role in the regulation of protein phosphatase activity in the nuclei of rat kidney cortex cells. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.1214","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T20:41:50Z","timestamp":1030308110000},"page":"111-120","source":"Crossref","is-referenced-by-count":7,"title":["Inhibitory effect of regucalcin on protein phosphatase activity in the nuclei of rat kidney cortex"],"prefix":"10.1002","volume":"83","author":[{"given":"Yoshiko","family":"Morooka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayoshi","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,8,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_5_3_1","first-page":"19","article-title":"Calmodulin plays a pivotal role in cellular regucalcin","volume":"202","author":"Cheung WY","year":"1984","journal-title":"Science"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.9.3686"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90041-S"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90405-0"},{"key":"e_1_2_5_7_1","first-page":"323","article-title":"Enhancement of protein tyrosine phosphatase activity in the proliferation of cloned rat hepatoma H4\u2010II\u2010E cells: suppressive role of endogenous regucalcin","volume":"6","author":"Inagaki S","year":"2000","journal-title":"Int J Mol Med"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"6398","DOI":"10.1016\/S0021-9258(18)83363-4","article-title":"Calcium activated DNA fragmentation in rat liver nuclei","volume":"264","author":"Jones DP","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19981215)71:4<569::AID-JCB11>3.0.CO;2-Z"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(96)90296-6"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006894332337"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006865507026"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006819507433"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.21.315"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"224","author":"Laemmli UK","year":"1970","journal-title":"Nature"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phoenol reagent","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.1098"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/1821441a0"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19991201)75:3<437::AID-JCB9>3.0.CO;2-W"},{"key":"e_1_2_5_20_1","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/S0021-9258(18)32091-X","article-title":"Calmodulin\u2010stimulated dephosphorylation of p\u2010nitrophenylphosphate and free phosphotyrosine by calcineurin","volume":"258","author":"Pallen CJ","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80884-J"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80998-A"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227541"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19990915)74:4<663::AID-JCB15>3.0.CO;2-2"},{"key":"e_1_2_5_25_1","first-page":"655","article-title":"Translocation of regucalcin to rat liver nucleus: involvement of nuclear protein kinase and protein phosphatase regulation","volume":"6","author":"Tsurusaki Y","year":"2000","journal-title":"Int J Mol Med"},{"key":"e_1_2_5_26_1","first-page":"67","volume-title":"Calcium modulators","author":"Vincenzi FE","year":"1982"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"22100","DOI":"10.1016\/S0021-9258(20)80653-X","article-title":"Identification of a nuclear localization sequence within the structure of the human interleukin\u20101\u03b1 precursor","volume":"268","author":"Wessendorf JHM","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_5_28_1","first-page":"295","article-title":"Stimulatory effect of regucalcin on mitochondrial ATP\u2010dependent calcium uptake activity in rat kidney cortex","volume":"80","author":"Xue JH","year":"2000","journal-title":"J Cell Biochem"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00602-5"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3359"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00925738"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227538"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.26.1915"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.1214","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.1214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T14:02:39Z","timestamp":1697464959000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.1214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8,6]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["10.1002\/jcb.1214"],"URL":"https:\/\/doi.org\/10.1002\/jcb.1214","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8,6]]}}}