{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:27:09Z","timestamp":1758634029668},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,9,25]],"date-time":"2001-09-25T00:00:00Z","timestamp":1001376000000},"content-version":"vor","delay-in-days":267,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The retinal pigment epithelium is uniquely suited to gene therapy that uses lipid\u2010mediated DNA transfer due to its high phagocytic activity in situ. We compared the relative efficacy of phagocytosis on the uptake of labeled plasmid vectors by retinal pigment epithelial and ciliary epithelial cells in vitro. Relative levels of endocytosis were then compared with the efficiency of marker transgene expression in these cells. Human retinal pigment epithelial and ciliary epithelial cells from a single donor were isolated and expanded in vitro. Polyplex\u2010mediated transfections were performed using a rhodamine\u2010labeled expression vector for green fluorescent protein. Rhodamine\u2010labeled endosomes were examined by fluorescence microscopy at different time points. Rhodamine labeling and green fluorescent protein expression were analyzed by flow cytometry 48 h after transfection. These gene transfer studies showed that expression of transgenes does occur in both human retinal pigment epithelial and ciliary epithelial cells in vitro. Endocytosis of labeled plasmid vectors occurs at a significantly higher number and density in retinal pigment epithelial cells than in ciliary epithelial cells (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.04). However, the efficiency of marker transgene expression is similar in the two cell types. These studies demonstrate that the higher intrinsic phagocytic activity does not enhance the efficacy of transgene expression in retinal pigment epithelial cells in vitro. Both human retinal pigment epithelial and ciliary epithelial cells are competent recipients for lipid\u2010mediated gene transfer, and transgene expression occurs at similar levels in both cell types. J. Cell. Biochem. 83: 671\u2013677, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.1258","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:15:54Z","timestamp":1030310154000},"page":"671-677","source":"Crossref","is-referenced-by-count":3,"title":["Comparative analysis of the uptake and expression of plasmid vectors in human ciliary and retinal pigment epithelial cells in vitro"],"prefix":"10.1002","volume":"83","author":[{"given":"Edward","family":"Chaum","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"2202","article-title":"Adenovirus\u2010mediated heme oxygenase\u20101 gene transfer into rabbit ocular tissues","volume":"36","author":"Abraham NG","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_3_1","first-page":"1462","article-title":"Efficiency of 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