{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T16:22:25Z","timestamp":1754151745924,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T00:00:00Z","timestamp":1095984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The maximum gene exhibition was shown to be achieved at 48 h after transfection with human <jats:italic>bcl\u20102<\/jats:italic> (h<jats:italic>bcl\u20102<\/jats:italic>) genes built in an SV40 early promoter\u2010based plasmid vector and HVJ\u2010liposome for cultured rat hepatocytes. The similar procedure of h<jats:italic>bcl\u20102<\/jats:italic> transfection was therefore conducted for livers in rats via the portal vein, and after 48 h followed by post\u2010ischemic reperfusion (I\/R) operation for some hepatic lobes. The I\/R\u2010induced hepatic injuries were in situ observed as both cell morphological degeneration and cellular DNA strand cleavages around capillary vessels of the ischemic liver lobes as detected by HE stain and TUNEL assay, and were biochemically observed as release of two hepatic marker enzymes AST and ALT into serum. All the I\/R\u2010induced injuries examined were appreciably repressed for rats transfected with h<jats:italic>bcl\u20102<\/jats:italic>; h<jats:italic>bcl\u20102<\/jats:italic> was expressed in hepatocytes around the capillaries of ischemic regions such as the median lobe and the left lobe, but scarcely around those of non\u2010ischemic regions. Thus cytoprotection against I\/R\u2010induced injuries may be attributed to the I\/R\u2010promoted expression of transferred h<jats:italic>bcl\u20102<\/jats:italic> genes. The possibility was examined firstly by methylphenylindole method, which showed that I\/R\u2010enhanced lipid peroxidation in the reference vector\u2010transfected livers were markedly repressed in the h<jats:italic>bcl\u20102<\/jats:italic>\u2010transfected livers. Contents of ascorbic acid (Asc) in serum and livers of h<jats:italic>bcl\u20102<\/jats:italic>\u2010transfected rats were enriched, unexpectedly, versus those of non\u2010transfected rats, and were as abundant as 1.90\u2010fold and 1.95\u2010 to 2.60\u2010fold versus those in the pre\u2010ischemic state, respectively. After I\/R, an immediate decline in serum Asc occurred in h<jats:italic>bcl\u20102<\/jats:italic>\u2010transfectants, and was followed by prompt restoration up to the pre\u2010ischemic Asc levels in contrast to the unaltered lower Asc levels in non\u2010transfectants except a transient delayed increase. Hepatic Asc contents were also diminished appreciably at the initial stage after I\/R in the ischemic lobes of h<jats:italic>bcl\u20102<\/jats:italic>\u2010transfectants, which however retained more abundant Asc versus non\u2010transfectants especially at the initial I\/R stage when scavenging of the oxidative stress should be most necessary for cytoprotection. The results showed a close correlation between cytoprotection by exogenously transferred h<jats:italic>bcl\u20102<\/jats:italic> and repressive effects on the lipid peroxidation associated with Asc consumption or redistribution. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20221","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T11:01:05Z","timestamp":1096023665000},"page":"857-870","source":"Crossref","is-referenced-by-count":4,"title":["Cytoprotection by <i>bcl\u20102<\/i> gene transfer against ischemic liver injuries together with repressed lipid peroxidation and increased ascorbic acid in livers and serum"],"prefix":"10.1002","volume":"93","author":[{"given":"Shinobu","family":"Yanada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasukazu","family":"Saitoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasufumi","family":"Kaneda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhioko","family":"Miwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199903270-00001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.16.7162"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90018-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10577"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(00)00573-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.15.1899"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80066-N"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)20008-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8235659"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1972.33"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199904270-00003"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0697-614"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.5897"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00311659"},{"issue":"3","key":"e_1_2_6_16_1","first-page":"537","article-title":"Role 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