{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T15:26:43Z","timestamp":1744817203362},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T00:00:00Z","timestamp":1095984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2004,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hyaline layer (HL) is an apically located extracellular matrix (ECM) which surrounds the sea urchin embryo from the time of fertilization until metamorphosis occurs. While gelatin\u2010cleavage activities were absent from freshly prepared hyaline layers, a dynamic pattern of activities developed in layers incubated at 15 or 37\u00b0C in Millipore\u2010filtered sea water (MFSW). Cleavage activities at 90, 55, 41, and 32 kDa were evident following incubation at either temperature. The activation pathway leading to the appearance of these species was examined to determine the minimum salt conditions required for processing and to establish precursor\u2013product relationships. In both qualitative and quantitative assays, the purified 55 kDa gelatinase activity was inhibited by 1,10\u2010phenanthroline (a zinc\u2010specific chelator) and ethylenebis (oxyethylenenitrilo) tetraacetic acid (EGTA). Calcium reconstituted the activity of the EGTA\u2010inhibited enzyme with an apparent dissociation constant (calcium) of 1.2 mM. Developmental substrate gel analysis was performed using various stage embryos. The 55 and 32 kDa species comigrated with gelatin\u2010cleavage activities present in sea urchin embryos. Collectively, the results reported here document a zymogen activation pathway which generates a 55 kDa, gelatin\u2010cleaving activity within the extraembryonic HL. This species displayed characteristics of the matrix metalloproteinase class of ECM modifying enzymes. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20268","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T15:01:03Z","timestamp":1096038063000},"page":"1075-1083","source":"Crossref","is-referenced-by-count":5,"title":["Zymogen activation and characterization of a major gelatin\u2010cleavage activity localized to the sea urchin extraembryonic matrix"],"prefix":"10.1002","volume":"93","author":[{"given":"Lavanya","family":"Ranganathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Rimsay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.117.4.1183"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1242\/dev.104.3.391","article-title":"Sea urchin morphogenesis and cell\u2010hyalin adhesion are perturbed by a monoclonal antibody specific to hyalin","volume":"104","author":"Adelson DC","year":"1988","journal-title":"Development"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90074-7"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2319"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1297-439"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc745"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(70)90257-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(71)90027-4"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/o00-024"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90338-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during assembly of the head of bacteriophage T4","volume":"272","author":"Laemmli UK","year":"1970","journal-title":"Nature"},{"key":"e_1_2_5_13_1","first-page":"211","article-title":"Purification and metal ion requirements of a candidate matrix metalloprotease: A 41 kDa gelatinase activity in the sea urchin embryo. Biochem","volume":"74","author":"Mayne J","year":"1996","journal-title":"Cell Biol"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-169X.2002.00645.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.9.2319"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90175-0"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1993.tb00655.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(93)90014-O"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1139\/o90-162"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(91)90114-X"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20010101)80:1<139::AID-JCB130>3.0.CO;2-A"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1139\/o01-034"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90351-7"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051990107"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080400106"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80429-8"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5392.1279"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90148-5"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90025-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1242\/dev.106.2.355","article-title":"Transcription of the spec\u20101 gene of Lytechinus is selectively inhibited in response to disruption of the extracellular matrix","volume":"106","author":"Wessel GM","year":"1989","journal-title":"Development"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.40.23730"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.20268","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.20268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:56:05Z","timestamp":1696798565000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,24]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,12,15]]}},"alternative-id":["10.1002\/jcb.20268"],"URL":"https:\/\/doi.org\/10.1002\/jcb.20268","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,24]]}}}