{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:03:57Z","timestamp":1790489037993,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,11,16]],"date-time":"2004-11-16T00:00:00Z","timestamp":1100563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have examined the expression of Thy\u20101, an abundant glycosylphosphatidylinositol (GPI)\u2010anchored glycoprotein, in dorsal root ganglia (DRG) and associated nerve fascicles, during postnatal development and following a nerve crush. The expression levels of Thy\u20101 in DRG neurons, dorsal roots, and central processes in spinal cord were rather low at postnatal day 2, and gradually increased as DRG neurons matured. During early development, the expression of Thy\u20101 within DRG neurons was low and equally distributed between plasma membrane and cytosol. With maturation, the staining intensities of Thy\u20101 in both the plasma membrane and the cytosol of DRG neurons became increased. We also studied Thy\u20101 expression in the regeneration of mature DRG neurons following the crush injury of sciatic nerve. Two days after the crush injury, Thy\u20101 expression dramatically decreased in the DRG neurons on the lesion side. Between 4 and 7 days after the injury, the expression of Thy\u20101 gradually increased and returned to a normal level 1 week after the sciatic nerve crush. The time course of the up\u2010regulation of Thy\u20101 expression during regeneration matched that of the recovery of sensory functions, such as pain withdraw reflex, placing reflex, and the score of Basso\u2013Beattie\u2013Bresnahan Locomotor Rating Scale. Taken together, our results suggest that Thy\u20101 expression is developmentally regulated and is closely associated with the functional maturation of DRG neurons during both postnatal development and nerve regeneration. Furthermore, perturbation of Thy\u20101 function with anti\u2010Thy\u20101 antibodies promoted neurite outgrowth from primary cultured DRG neurons, again confirming the inhibitory role of Thy\u20101 on neurite outgrowth. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20341","type":"journal-article","created":{"date-parts":[[2004,11,16]],"date-time":"2004-11-16T15:46:44Z","timestamp":1100620004000},"page":"684-694","source":"Crossref","is-referenced-by-count":26,"title":["Role of Thy\u20101 in in vivo and in vitro neural development and regeneration of dorsal root ganglionic neurons"],"prefix":"10.1002","volume":"94","author":[{"given":"Chien\u2010Hsing","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seu\u2010Mei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Hung","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung\u2010Jiuan","family":"Jeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1994.1001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-97602002000200015"},{"key":"e_1_2_5_4_1","first-page":"457","article-title":"Activation of T lymphocytes by cross\u2010linking of glycophospholipid\u2010anchored Thy\u20101 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