{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:21:31Z","timestamp":1773289291947,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasopharyngeal carcinoma (NPC) is a common cancer in South China but is rare in other parts of the world. A novel NPC\u2010related gene was isolated by location candidate cloning strategy, whose expression was down\u2010regulated in NPC. This gene was designated human NGX6 (Genbank accession AF188239) and encoded a predicted protein of 338 amino acids that harbors an EGF\u2010like domain. The effects of NGX6 on cells from human NPC cell line HNE1 were investigated. The cells transfected with NGX6 had a markedly high expression of NGX6, leading to significant decrease in cell proliferation and the capability to form colonies in soft agar, delaying the G<jats:sub>0<\/jats:sub>\u2013G<jats:sub>1<\/jats:sub> cell cycle progression. Flow cytometry assay indicated that the expression of cyclin D1 significantly decreased in NGX6\u2010transfected HNE1 cells as well as cyclin A and E. There was a delay in tumor formation and a dramatic reduction in tumor size when cells transfected with NGX6 were injected into nude mice. In another way, we found NGX6 played a negative role in EGFR Ras\/Mek\/MAPK pathway. We propose that NGX6, as an EGF\u2010like domain gene, could delay cell cycle G<jats:sub>0<\/jats:sub>\u2013G<jats:sub>1<\/jats:sub> progression and thus inhibit cell proliferation by negatively regulating EGFR pathway in NPC cells and down\u2010regulating the expression of cyclin D1 and E. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20393","type":"journal-article","created":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T23:17:19Z","timestamp":1108768639000},"page":"64-73","source":"Crossref","is-referenced-by-count":23,"title":["<i>NGX6<\/i> gene inhibits cell proliferation and plays a negative role in EGFR pathway in nasopharyngeal carcinoma 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