{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:32:52Z","timestamp":1791919972714,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,29]],"date-time":"2005-06-29T00:00:00Z","timestamp":1120003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parathyroid hypertensive factor (PHF) is a novel substance secreted by the parathyroid gland (PTG), which is elevated in 30\u201340% of all hypertensive patients; specifically, the low\u2010renin subset. However, very little is known about the regulation of PHF secretion. Since the classical parathyroid regulator, 1,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> (1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>), may be elevated concurrent with or preceding the development of low\u2010renin hypertension and elevated plasma PHF, we hypothesized that 1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> would stimulate PHF release. To test this hypothesis, PTG organ and cell cultures, derived from spontaneously hypertensive rats (SHR) and the normotensive genetic control Wistar Kyoto (WKY) rats, were exposed to various vitamin D<jats:sub>3<\/jats:sub> metabolites and PHF release measured by ELISA. 1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> rapidly stimulated PHF release with enhanced sensitivity in SHR versus WKY cultures indicated by a leftward shift in the dose\u2010response curve, whereas 24,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> (24,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>) had the converse effect. Vitamin D<jats:sub>3<\/jats:sub> analog \u201cBT,\u201d an agonist for the classical nuclear vitamin D receptor (1,25VDR<jats:sub>nuc<\/jats:sub>), was without effect suggesting a 1,25VDR<jats:sub>nuc<\/jats:sub>\u2010independent mechanism and potential involvement of the plasma membrane\u2010bound vitamin D receptor (1,25 D<jats:sub>3<\/jats:sub>\u2010MARRS). Interestingly, protein expression of the 1,25 D<jats:sub>3<\/jats:sub>\u2010MARRS was increased in SHR versus WKY parathyroid cells. In conclusion, these results support the idea that 1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> may contribute to elevated plasma PHF in the SHR. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20528","type":"journal-article","created":{"date-parts":[[2005,6,29]],"date-time":"2005-06-29T23:22:04Z","timestamp":1120087324000},"page":"97-108","source":"Crossref","is-referenced-by-count":3,"title":["Regulation of parathyroid hypertensive factor secretion by vitamin D<sub>3<\/sub> analogs in parathyroid cells derived from spontaneously hypertensive rats"],"prefix":"10.1002","volume":"96","author":[{"given":"S.K.","family":"Sutherland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Nemere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.G.","family":"Benishin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(99)00173-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-6-2738"},{"key":"e_1_2_6_4_1","first-page":"F104","article-title":"Developmental changes of Ca2+, PO4, and calcitriol metabolism in spontaneously hypertensive rats","volume":"259","author":"Bourgouin P","year":"1990","journal-title":"Am J Physiol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(01)00160-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1210\/endo.130.1.1309332","article-title":"Binding and metabolism of 1,25\u2010dihydroxyvitamin D3 in cultured bovine parathyroid cells","volume":"130","author":"Brown AJ","year":"1992","journal-title":"Endocrinology"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/2.7.553"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.10.6203"},{"key":"e_1_2_6_9_1","first-page":"2211","article-title":"Vitamin D: The vitamin and the hormone","volume":"33","author":"DeLuca HF","year":"1974","journal-title":"Fed Proc"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.1979.tb06660.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.3.3280376"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8097"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199006000-00003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-112-1-200"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-4-1876"},{"issue":"1","key":"e_1_2_6_16_1","first-page":"S154","article-title":"1,25(OH)2 vitamin D3\u2010induced alterations in vascular reactivity in the spontaneously hypertensive rat","volume":"307","author":"Hatton DC","year":"1994","journal-title":"Am J Med Sci"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(75)90391-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-113-6-1950"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0021-9258(17)38111-5","article-title":"1,25\u2010dihydroxyvitamin D3 receptors in parathyroid glands. 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