{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:03:54Z","timestamp":1761487434009},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T00:00:00Z","timestamp":1123459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2005,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> infection results in peptic ulceration and chronic gastritis through mechanisms which are not fully elucidated. Live <jats:italic>H. pylori<\/jats:italic> activate the pro\u2010inflammatory transcription factor NF\u2010\u03baB in gastric epithelial cells. Patients may have peptic ulcer disease in the absence of <jats:italic>H. pylori<\/jats:italic> infection; therefore other factors contribute to the inflammatory process. Maximal acid output in patients with <jats:italic>H. pylori<\/jats:italic> infection and duodenal ulceration is significantly increased indicating a role for acid in the pathogenesis of mucosal ulceration. The effect of low pH on NF\u2010\u03baB activation in gastric epithelial cells has not been studied. Human gastric epithelial cells (AGS) were exposed to a range of pH changes in the presence or absence of <jats:italic>H. pylori<\/jats:italic>. NF\u2010\u03baB DNA\u2010binding and cytosolic I\u03baB\u2010\u03b1 were measured using electrophoretic mobility shift assay and Western blotting. NF\u2010\u03baB DNA\u2010binding in gastric epithelial cells dramatically increased when the pH of the culture medium decreased. Increases in NF\u2010\u03baB nuclear binding were paralleled by decreasing amounts of cytosolic I\u03baB\u2010\u03b1. These findings were similar but less potent than those observed when cells were exposed to <jats:italic>H. pylori<\/jats:italic>. Low pH resulted in enhancement of <jats:italic>H. pylori<\/jats:italic>\u2010induced NF\u2010\u03baB nuclear binding. DNA binding of NF\u2010\u03baB activation secondary to low pH was attenuated by PD98059 but not by SB203580. Similar to <jats:italic>H. pylori<\/jats:italic>, low pH potently and independently augments NF\u2010\u03baB nuclear binding in AGS cells and such activation appears to be mediated through MEK1\u2010dependant signaling pathways. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20539","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T22:28:58Z","timestamp":1123540138000},"page":"589-598","source":"Crossref","is-referenced-by-count":14,"title":["Low pH and <i>Helicobacter pylori<\/i> increase nuclear factor kappa B binding in gastric epithelial cells: A common pathway for epithelial cell injury?"],"prefix":"10.1002","volume":"96","author":[{"given":"Dermot","family":"O'Toole","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed M.M.","family":"Abdel\u2010Latif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aideen","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry J.","family":"Windle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Bowie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke A.J.","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald G.","family":"Weir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dermot","family":"Kelleher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,8,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"63","article-title":"The inducible transcription factor NF\u2010\u03baB: Regulation by distinct protein subunits","volume":"1072","author":"Baeuerle P","year":"1991","journal-title":"Biochem Biophys Acta"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001041"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.11.10.4943"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5288.782"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.9.5086"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00542.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/360458a0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90126-J"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200109)31:9<2829::AID-IMMU2829>3.0.CO;2-U"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(94)00186-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)80010-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5217"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00360-1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.32.12.1473"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/41493"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02996319"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70632-8"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1128\/IAI.66.5.2346-2348.1998","article-title":"Proteins encoded by the cag pathogenicity island of Helicobacter pylori are required for NF\u2010\u03baB activation","volume":"66","author":"Glocker E","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3965-3971.2001"},{"key":"e_1_2_6_22_1","first-page":"32","volume-title":"Practical immunology","author":"Hudson L","year":"1989"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9322504"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.10.5552"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2000.5866"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81842-5"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(02)02002-2"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5339.818"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000959200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.1.18"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.33.3.289"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.4049\/jimmunol.153.6.2681","article-title":"Activation of NF\u2010kappa B and induction of vascular cell adhesion molecule\u20101 and intracellular adhesion molecule\u20101 expression in human glial cells by IL\u20101. Modulation by antioxidants","volume":"153","author":"Moynagh PN","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"6140","DOI":"10.4049\/jimmunol.159.12.6140","article-title":"A secreted\/shed product of Helicobacter pylori activates transcription factor nuclear factor\u2010kappa B","volume":"159","author":"Munzenmaier A","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.44.31655"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0996-998"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7399"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.7.2336"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7420"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0011-2240(02)00018-4"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.4.477"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)02011-Z"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/10799900050198471"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49611-7"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9921079"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.10.2645"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5339.866"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108347200"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.20539","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.20539","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T19:15:32Z","timestamp":1696792532000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,8]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,10,15]]}},"alternative-id":["10.1002\/jcb.20539"],"URL":"https:\/\/doi.org\/10.1002\/jcb.20539","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,8]]}}}