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Our study was undertaken to investigate the effect of changes in glucose and insulin concentrations on adenosine metabolism, transport and receptor\u2010mediated action in rat T lymphocytes. Presented results indicate that vulnerability of T cells to metabolic stress is determined by insulin but not by glucose concentration. However, glucose and insulin differentially affected the activities of adenosine metabolizing enzymes in resting and proliferating T cells. The Con A\u2010induced proliferation of cultured T lymphocytes did not depended on expression level and functional state of nucleoside transporters. Inhibition of adenosine kinase (AK) with 5\u2010iodotubercidin lowers the proliferation potential of T cells to the level observed for insulin\u2010deprived cells. Moreover, insulin\u2010deprived T lymphocytes but not cells cultured in the presence of insulin released significant quantities of adenosine. Under resting conditions, the cAMP level was fivefold higher in cells deprived of insulin comparing to cells cultured in the presence of insulin. Exposition of insulin\u2010deprived T lymphocytes to specific antagonist (ZM241385) of A2a receptor but not to specific antagonist (Alloxazine) of A2b receptor suppressed cAMP elevation and completely restored the proliferation potential of T cells. Concluding, adenosine released by insulin\u2010deprived T cells due to suppressed AK activity by acting on A2a receptors leads to increases in cAMP level and suppression of T cell proliferation. We assume that this mechanism may significantly contribute to immune impairment observed in diabetes. J. Cell. Biochem. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20642","type":"journal-article","created":{"date-parts":[[2005,9,19]],"date-time":"2005-09-19T20:46:40Z","timestamp":1127162800000},"page":"1296-1310","source":"Crossref","is-referenced-by-count":6,"title":["Differential effect of insulin and elevated glucose level on adenosine handling in rat T lymphocytes"],"prefix":"10.1002","volume":"96","author":[{"given":"Tadeusz","family":"Pawelczyk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Sakowicz\u2010Burkiewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Kocbuch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Szutowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,9,19]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.4049\/jimmunol.155.6.2813","article-title":"Adenosine acts as an endogenous modulator of IL\u20102\u2010dependent proliferation of cytotoxic T lymphocytes","volume":"155","author":"Antonysamy MA","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1995.tb00680.x"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(87)90333-X"},{"key":"e_1_2_4_5_1","doi-asserted-by":"crossref","first-page":"15738","DOI":"10.1016\/S0021-9258(18)55459-4","article-title":"Selective adenosine release from human B but not T lymphoid cell line","volume":"265","author":"Barankiewicz J","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_4_6_1","first-page":"218","article-title":"Pharmacological modification of immunoregulatory T lymphocytes. 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