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To characterize the function of mouse<jats:italic>\u03b1\u2010internexin<\/jats:italic>promoter, we designed two different expression constructs driven by 0.7 kb or 1.3 kb of mouse<jats:italic>\u03b1\u2010internexin<\/jats:italic>5\u2032\u2010flanking sequences; one was the enhanced green fluorescent protein (EGFP) reporter for monitoring specific expression in vitro, and the other was the<jats:italic>cre<\/jats:italic>for studying the functional DNA recombinase in transgenic mice. After introducing DNA constructs into non\u2010neuronal 3T3 fibroblasts and a neuronal Neuro2A cell line by lipofectamine transfection, we observed that the expression of EGFP with 1.3 kb mouse<jats:italic>\u03b1\u2010internexin<\/jats:italic>promoter was in a neuron\u2010dominant manner. To establish a tissue\u2010specific pattern in the nervous system, we generated a transgenic mouse line expressing Cre DNA recombinase under the control of 1.3 kb<jats:italic>\u03b1\u2010Internexin<\/jats:italic>promoter. The activity of the Cre recombinase at postnatal day 1 was examined by mating the<jats:italic>cre<\/jats:italic>transgenic mice to ROSA26 reporter (R26R) mice with knock\u2010in Cre\u2010mediated recombination. Analyses of postnatal day 1 (P1) newborns showed that \u03b2\u2010galactosidase activity was detected in the peripheral nervous system (PNS), such as cranial nerves innervating the tongue and the skin as well as spinal nerves to the body trunk. Furthermore, X\u2010gal\u2010labeled dorsal root ganglionic (DRG) neurons showed positive for \u03b1\u2010Internexin in cell bodies but negative in their spinal nerves. The motor neurons in the spinal cord did not exhibit any \u03b2\u2010galactosidase activity. Therefore, the<jats:italic>cre<\/jats:italic>transgene driven by mouse<jats:italic>\u03b1\u2010internexin<\/jats:italic>promoter, described here, provides a useful animal model to specifically manipulate genes in the developing nervous system. J. Cell. Biochem. 97: 275\u2013287, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20643","type":"journal-article","created":{"date-parts":[[2005,9,19]],"date-time":"2005-09-19T21:46:58Z","timestamp":1127166418000},"page":"275-287","source":"Crossref","is-referenced-by-count":5,"title":["Neural expression of<i>\u03b1\u2010internexin<\/i>promoter in vitro and in 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