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Specifically, the capabilities of the label\u2010free photothermal (PT) technique for detecting and imaging cells in the microvessel network of rat mesentery are analyzed from the point of view of overcoming the problems of flow cytometry in vivo. These problems include, among others, the influences of light scattering and absorption in vessel walls and surrounding tissues, instability of cell velocity, and cells numbers and positions in a vessel's cross\u2010section. The potential applications of this new approach in cell biochemistry and medicine are discussed, including molecular imaging; studying the metabolism and pathogenesis of many diseases at a cellular level; and monitoring and quantifying metastatic and apoptotic cells, and\/or their responses to therapeutic interventions (e.g., drug or radiation), in natural biological environments. J. Cell. Biochem. 97: 916\u2013932, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20766","type":"journal-article","created":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T23:17:24Z","timestamp":1137021444000},"page":"916-932","source":"Crossref","is-referenced-by-count":55,"title":["In vivo photothermal flow cytometry: Imaging and detection of individual cells in blood and lymph flow"],"prefix":"10.1002","volume":"97","author":[{"given":"Vladimir P.","family":"Zharov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina I.","family":"Galanzha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valery V.","family":"Tuchin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"G549","article-title":"Variations in 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