{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:39:27Z","timestamp":1784828367996,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,26]],"date-time":"2006-01-26T00:00:00Z","timestamp":1138233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2006,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Various studies indicate that periodontal ligament fibroblasts (PLF) have some similarities to osteoblasts, for example they have the capacity to induce the formation of osteoclast\u2010like cells. Here, we investigated whether a second population of tooth\u2010associated fibroblasts, gingival fibroblasts (GF), has similar osteoclastogenesis properties. PLF and GF were co\u2010cultured with peripheral blood mononuclear cells (PBMC) in the presence and absence of dexamethasone and 1\u03b1,25dihydroxycholecalciferol (dex\u2009+\u2009vit D<jats:sub>3<\/jats:sub>) on plastic and on cortical bone slices. Tartrate resistant acid phosphatase (TRACP) positive multinucleated cells (MNCs) were more abundant in co\u2010cultures with PLF than in GF\u2010PBMC co\u2010cultures, more abundant on plastic compared to bone and more abundant in the presence of dex\u2009+\u2009vit D<jats:sub>3<\/jats:sub>. In line with these findings was an inhibition of MNC formation and not inhibition of existing osteoclasts by medium conditioned by GF. We next investigated whether expression of molecules important for osteoclastogenesis differed between the two types of fibroblasts and whether these molecules were regulated by dex\u2009+\u2009vit D<jats:sub>3<\/jats:sub>. OPG was detected at high levels in both fibroblast cultures, whereas RANKL could not be detected. Resorption of bone did not occur by the MNCs formed in the presence of either fibroblast subpopulation, suggesting that the fibroblasts secrete inhibitors of bone resorption or that the osteoclast\u2010like cells were not functional. The incapacity of the MNCs to resorb was abolished by culturing the fibroblast\u2010PBMC cultures with M\u2010CSF and RANKL. Our results suggest that tooth\u2010associated fibroblasts may trigger the formation of osteoclast\u2010like cells, but more importantly, they play a role in preventing bone resorption, since additional stimuli are required for the formation of active osteoclasts. J. Cell. Biochem. 98: 370\u2013382, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20795","type":"journal-article","created":{"date-parts":[[2006,1,26]],"date-time":"2006-01-26T21:21:14Z","timestamp":1138310474000},"page":"370-382","source":"Crossref","is-referenced-by-count":83,"title":["Gingival fibroblasts are better at inhibiting osteoclast formation than periodontal ligament fibroblasts"],"prefix":"10.1002","volume":"98","author":[{"given":"Teun J.","family":"de Vries","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton","family":"Schoenmaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nutthamon","family":"Wattanaroonwong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marije","family":"van den 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