{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:02:01Z","timestamp":1758628921641},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T00:00:00Z","timestamp":1151625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2006,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous work in our laboratory has shown agkistin, a snake venom metalloproteases (SVMPs) from the venom of <jats:italic>Agkistrodon halys<\/jats:italic>, possesses antiplatelet aggregation activity. In this study, we further examined the antiangiogenic activity of agkistin\u2010s, the disintegrin domain of agkistin. Recombinant agkistin\u2010s was produced in <jats:italic>Escherichia coli<\/jats:italic> by subcloning its cDNA into pET28a vector, and the effect of purified agkistin\u2010s was evaluated. At the concentration of 0.5\u20131.5 \u00b5M, the recombinant agkistin\u2010s exhibited inhibitory activities on the bovine aortic endothelial cells (BAECs) migration and proliferation in a dose\u2010dependent manner. In addition, it exhibited an effective antiangiogenic effect when assayed by using the 10\u2010day\u2010old embryo chick CAM model and effectively inhibits the tube\u2010like structure formation. Furthermore, it potently induced BAECs apoptosis as examined by flow cytometric assays. J. Cell. Biochem. 99: 1517\u20131523, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20859","type":"journal-article","created":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T09:02:44Z","timestamp":1156237364000},"page":"1517-1523","source":"Crossref","is-referenced-by-count":13,"title":["Agkistin\u2010s, a disintegrin domain, inhibits angiogenesis and induces BAECs 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