{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:15:28Z","timestamp":1760897728959,"version":"3.32.0"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2006,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We reported recently that roscovitine (ROSC), a selective cyclin\u2010dependent kinase (CDK) inhibitor, arrested human MCF\u20107 breast cancer cells in G<jats:sub>2<\/jats:sub>phase of the cell cycle and concomitantly induced apoptosis. On the other hand, ROSC\u2010induced G<jats:sub>1<\/jats:sub>arrest observed by another group has not been accompanied by apoptosis. Therefore, we decided to prove to which extent components of tissue culture media could affect the primary action of ROSC. For this purpose we compared the efficacy of the ROSC treatment on MCF\u20107 cells cultivated in medium with and without phenol red. The kinetics of MCF\u20107 cell proliferation strongly depended on the presence of phenol red that has been recognized previously as a weak estrogen. Exposure of MCF\u20107 cells cultivated in phenol red\u2010deprived medium to ROSC resulted in a strong G<jats:sub>2<\/jats:sub>arrest and apoptosis. However, the anti\u2010proliferative and pro\u2010apoptotic action of ROSC was strongly diminished in cells maintained in medium containing phenol red. The ratio of the G<jats:sub>2<\/jats:sub>cell population after 12 h ROSC was reduced by approximately 20% in the latter and correlated with the lack of CDK2 inactivation. Moreover, the kinetics of ROSC\u2010induced apoptosis was delayed in the presence of phenol red. These results clearly evidence that the efficacy of the therapy of ER\u2010positive breast cancers by CDK inhibitors is diminished in the presence of estrogen\u2010mimicking compounds and indicate that phytoestrogens and xenoestrogens could interfere with the therapy. Therefore, the exposure of cancer patients to the estrogen mimics should be avoided at least during chemotherapy by CDK inhibitors. J. Cell. Biochem. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.20960","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T22:03:52Z","timestamp":1149199432000},"page":"1367-1379","source":"Crossref","is-referenced-by-count":17,"title":["Phenol red reduces ROSC mediated cell cycle arrest and apoptosis in human MCF\u20107 cells"],"prefix":"10.1002","volume":"98","author":[{"given":"J\u00f3zefa","family":"W\u0119sierska\u2010G\u0105dek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Schreiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marieta","family":"Gueorguieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Ranftler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2713"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-200201000-00015"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.8.2496"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10158"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401610"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.1.1.102"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.1.4.21"},{"key":"e_1_2_6_9_1","first-page":"4364","article-title":"Down\u2010regulation of cyclin D1 by transcriptional repression in MCF\u20107 human breast carcinoma cells induced by flavopiridol","volume":"59","author":"Carlson B","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd773"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203103"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204741"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.0518a.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.14.4.457"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(00)22023-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.9.2777"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986730609220401152358"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/320134a0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","unstructured":"GreeneGL GilnaP WaterfieldM BakerA HortY ShineJ.1986.Sequence and expression of human estrogen receptor complementary DNA. Science231:1150\u20131154.","DOI":"10.1126\/science.3753802"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403295"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81683-9"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9357"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.9.3379"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000944"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203780"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5925"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0026-895X(24)12306-1","article-title":"The cyclin\u2010dependent kinase inhibitor roscovitine inhibits RNA synthesis and triggers nuclear accumulation of p53 that is unmodified at Ser15 and Lys382","volume":"60","author":"Ljungman M","year":"2001","journal-title":"Mol Pharmacol"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204412"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.14.1126"},{"key":"e_1_2_6_30_1","first-page":"2883","article-title":"p53AIP1 regulates the mitochondrial apoptotic pathway","volume":"62","author":"Matsuda K","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2004.03.006"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.t01-2-00527.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7545954"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00782-X"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80476-6"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00073-8"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2004.02.001"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00232a015"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068537"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.12.1501"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1161"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030503"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1087"},{"key":"e_1_2_6_44_1","first-page":"895","article-title":"Dual action of cyclin\u2010dependent kinase inhibitors: induction of cell cycle arrest and apoptosis. A comparison of the effects exerted by roscovitine and cisplatin","volume":"55","author":"Wesierska Gadek J","year":"2003","journal-title":"Polish J Pharmacol"},{"key":"e_1_2_6_45_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1158\/1535-7163.113.4.1","article-title":"Roscovitine.induced up\u2010regulation of p53AIP1 protein precedes the onset of apoptosis inhuman MCF\u20107 breast cancer cells","volume":"4","author":"Wesierska Gadek J","year":"2005","journal-title":"Mol Cancer Ther"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20010101)80:1<85::AID-JCB80>3.0.CO;2-1"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.15.1.23"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20000915)78:4<681::AID-JCB17>3.0.CO;2-D"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10580"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3410"},{"key":"e_1_2_6_51_1","first-page":"15","article-title":"Role of p53 tumor suppressor in ageing: regulation of transient cell cycle arrest and terminal senescence","volume":"56","author":"Wesierska\u2010Gadek J","year":"2005","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20531"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11290"},{"key":"e_1_2_6_54_1","first-page":"53","article-title":"Activation of p53 protein in normal and tumor cells by a novel anticancer agent CHS 828","volume":"29","author":"Wojciechowski J","year":"2003","journal-title":"Drugs Exptl Clin Res"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8091231"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.20960","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.20960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T05:02:57Z","timestamp":1736398977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.20960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,8,15]]}},"alternative-id":["10.1002\/jcb.20960"],"URL":"https:\/\/doi.org\/10.1002\/jcb.20960","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"type":"print","value":"0730-2312"},{"type":"electronic","value":"1097-4644"}],"subject":[],"published":{"date-parts":[[2006,6]]}}}