{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:35:12Z","timestamp":1767857712064,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,8,3]],"date-time":"2006-08-03T00:00:00Z","timestamp":1154563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In late pregnancy rapidly increasing fetal growth dramatically increases uterine wall tension. This process has been implicated in the activation of the myometrium for labor, but the mechanisms involved are unclear. Here, we tested, using a rat model, the hypothesis that gestation\u2010dependent stretch, via activation of focal adhesion signaling, contributes to the published activation of myometrial ERK at the end of pregnancy. Consistent with this hypothesis, we show here that ERK is targeted to adhesion plaques during late pregnancy. Furthermore, myometrial stretch triggers a dramatic increase in myometrial contractility and ERK and caldesmon phosphorylation, confirming the presence of stretch sensitive myometrial signaling element. Screening by anti\u2010phosphotyrosine immunoblotting for focal adhesion signaling in response to stretch reveals a significant increase in the tyrosine phosphorylated bands identified as focal adhesion kinase (FAK), A\u2010Raf, paxillin, and Src. Pretreatment with PP2, a Src inhibitor, significantly suppresses the stretch\u2010induced increases in FAK, paxillin, Src, ERK and caldesmon phosphorylation and myometrial contractility. Thus, focal adhesion\u2010Src signaling contributes to ERK activation and promotes contraction in late pregnancy. These results point to focal adhesion signaling molecules as potential targets in the modulation of the myometrial contractility and the onset of labor. J. Cell. Biochem. 100: 129\u2013140, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21033","type":"journal-article","created":{"date-parts":[[2006,8,3]],"date-time":"2006-08-03T20:46:43Z","timestamp":1154638003000},"page":"129-140","source":"Crossref","is-referenced-by-count":30,"title":["Focal adhesion signaling is required for myometrial ERK activation and contractile phenotype switch before labor"],"prefix":"10.1002","volume":"100","author":[{"given":"Yunping","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Gallant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabah","family":"Malek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen G.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.12.1.463"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.21.5.514"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1080\/j.0001-6349.2005.00484.x","article-title":"Normal labor associated with changes in uterine heparan sulfate proteoglycan expression and localization","volume":"84","author":"Cluff AH","year":"2005","journal-title":"Acta Obstet Gynecol Scand"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.4.C1081"},{"key":"e_1_2_6_6_1","first-page":"893","volume-title":"Williams Obstetrics. 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