{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:49:41Z","timestamp":1757702981882},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,3,19]],"date-time":"2007-03-19T00:00:00Z","timestamp":1174262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To reveal growth factor and its signal pathway to CCAAT\/enhancer binding protein alpha (C\/EBP\u03b1) in hepatocyte differentiation, we used Huh\u20106 and HepG2, human hepatoblastoma (HBL) cell lines that maintain the expression of genes in hepatoblasts and remain at that stage of differentiation. Insulin\u2010like growth factor (IGF)\u2010II, hepatocyte growth factor (HGF), and dexamethasone (Dex) stimulated HBL cells for Northern blot analysis. Bromodeoxyuridine (BrdU) up\u2010take assay and Western blot analysis on albumin was performed to unveil proliferation and differentiation activity of IGF\u2010II. C\/EBP\u03b1 and phosphorylation of Akt were analyzed by Western blot analysis. LY294002 and wortmannin, specific inhibitors of PI3 kinase, and PD98059, a specific inhibitor of mitogen\u2010activated protein (MAP) kinase, were used to examine the signaling pathway of C\/EBP\u03b1 upregulated by IGF\u2010II. Luciferase assay was performed to study the promoter activity of C\/EBP\u03b1. Actinomycin D was used to analyze half\u2010life of C\/EBP\u03b1 mRNA. IGF\u2010II up\u2010regualted C\/EBP\u03b1 by Northern blot and Western blot while HGF and Dex did not by Northern blot. IGF\u2010II promoted proliferation and differentiation by BrdU up\u2010take assay and Western blot analysis on albumin. Akt phosphorylated by IGF\u2010II, suggested that phosphatidyl\u2010inositol (PI) 3 kinase mediated the signaling pathway of IGF\u2010II. LY294002 and wortmannin suppressed expression of C\/EBP\u03b1. IGF\u2010II activated the promoter activity and prolonged half\u2010life of mRNA, suggesting that IGF\u2010II activated promoter and stabilized mRNA. LY294002 and wortmannin suppressed the promoter activity of C\/EBP\u03b1 while PD98059 did not, suggesting that activation of the promoter was mediated by PI3 kinase. J. Cell. Biochem. 102: 161\u2013170, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21293","type":"journal-article","created":{"date-parts":[[2007,3,19]],"date-time":"2007-03-19T21:15:21Z","timestamp":1174338921000},"page":"161-170","source":"Crossref","is-referenced-by-count":8,"title":["Insulin\u2010like growth factor (IGF)\u2010II regulates CCAAT\/enhancer binding protein \u03b1 expression via phosphatidyl\u2010inositol 3 kinase in human hepatoblastoma cell lines"],"prefix":"10.1002","volume":"102","author":[{"given":"Minoru","family":"Tomizawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromitsu","family":"Saisho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"13144","DOI":"10.1016\/S0021-9258(18)69282-8","article-title":"Developmental regulation of insulin\u2010like growth factor II mRNA in different rat tissues","volume":"261","author":"Brown AL","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90513-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.02.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1967)20:3<396::AID-CNCR2820200308>3.0.CO;2-O"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4309"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1242\/dev.109.2.473","article-title":"Fetal and adult localization of C\/EBP: Evidence for combinatorial action of transcription factors in cell\u2010specific gene expression","volume":"109","author":"Kuo CF","year":"1990","journal-title":"Development"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(03)00159-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1490117"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2003.10.018"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35046522"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.07.011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.131.4.2030"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00459"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2613.1998.00082.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20629"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.3.1192"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8999"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.4.1325-1338.2005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1183304"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.21293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.21293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T02:38:51Z","timestamp":1697164731000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.21293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,19]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/jcb.21293"],"URL":"https:\/\/doi.org\/10.1002\/jcb.21293","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,19]]}}}