{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:52:55Z","timestamp":1789213975755,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,7,31]],"date-time":"2007-07-31T00:00:00Z","timestamp":1185840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study addresses whether alterations in osteoclasts (OCs) derived from <jats:italic>oim\/oim<\/jats:italic> mice, an established model of moderate\u2010to\u2010severe OI, are present. Bone marrow cells from <jats:italic>oim\/oim<\/jats:italic> and wildtype (+\/+) mice were cultured on bone slices in the presence of MCSF and RANKL and evaluated at days 0, 1, 2, 4, and 7. OCs were identified by tartrate\u2010resistant acid phosphatase (TRAP) staining, and bone slice resorption pits were analyzed by reflection microscopy. Flow cytometry was used to examine CD51 (integrin \u03b1V) and CD61 (integrin \u03b23) markers. Confocal microscopy was used to assess changes in OC morphology and resorption. There was no difference between the OC precursors of the two genotypes in expression of CD51 and CD61 markers. At day 2, the bone slices seeded with <jats:italic>oim\/oim<\/jats:italic> cells had a greater percentage of mononuclear cells associated with resorption pits compared to +\/+ bone slices. At day 4, the diameter and area of <jats:italic>oim\/oim<\/jats:italic> OCs were larger compared to the +\/+ OCs, and the number of nuclei per OC was also greater for the <jats:italic>oim\/oim<\/jats:italic> group. At day 7, the <jats:italic>oim\/oim<\/jats:italic> OCs contained more F\u2010actin rings compared to the +\/+ OCs, and the number of OCs in the <jats:italic>oim\/oim<\/jats:italic> group was greater compared to the +\/+ group. The resorbed area of bone slices for the <jats:italic>oim\/oim<\/jats:italic> group was also greater compared to the +\/+ group at day 7. In conclusion, <jats:italic>oim\/oim<\/jats:italic> mononuclear resorbing cells and OCs showed cellular changes and greater resorptive activity compared to +\/+ cells, features that likely contribute to dysregulated bone remodeling in OI. J. Cell. 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