{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:26:47Z","timestamp":1791919607432,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,21]],"date-time":"2007-05-21T00:00:00Z","timestamp":1179705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is controversially discussed that irradiation induces bystander effects via gap junction channels and\/or diffusible cellular factors such as nitric oxide or cytokines excreted from the cells into the environment. But up to now the molecular mechanism leading to a bystander response is not well understood. To discriminate between both mechanisms of bystander response, (i) mediated by gap junctional communication and\/or (ii) mediated by diffusible molecules, we used non\u2010communicating Jeg3 malignant trophoblast cells transfected with inducible gap junction proteins, connexin43 and connexin26, respectively, based on the Tet\u2010On system. We co\u2010cultivated X\u2010ray irradiated and non\u2010irradiated bystander Jeg3 cells for 4 h, separated both cell populations by flow cytometry and evaluated the expression of activated p53 by Western blot analysis. The experimental design was proven with communicating versus non\u2010communicating Jeg3 cells. Interestingly, our results revealed a bystander effect which was independent from gap junctional communication properties and the connexin isoform expressed. Therefore, it seems more likely that the bystander effect is not mediated via gap junction channels but rather by paracrine mechanisms via excreted molecules in Jeg3 cells. J. Cell. Biochem. 103: 149\u2013161, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21395","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T23:52:13Z","timestamp":1180223533000},"page":"149-161","source":"Crossref","is-referenced-by-count":25,"title":["Radiation\u2010induced bystander effects in malignant trophoblast cells are independent from gap junctional communication"],"prefix":"10.1002","volume":"103","author":[{"given":"Ferya","family":"Banaz\u2010Ya\u015far","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Lennartz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Winterhager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gellhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/3579865"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.011417098"},{"key":"e_1_2_6_4_1","first-page":"5436","article-title":"Oxidative metabolism modulates signal transduction and micronucleus formation in bystander cells from alpha\u2010particle\u2010irradiated normal human fibroblast cultures","volume":"62","author":"Azzam EI","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_1","first-page":"7128","article-title":"Expression of CONNEXIN43 is highly sensitive to ionizing radiation and other environmental stresses","volume":"63","author":"Azzam EI","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206961"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1191\/0960327104ht418oa"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15419060500514101"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1667\/0033-7587(2001)155[0335:FRIAGJ]2.0.CO;2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:CANC.0000031764.81141.e4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2005.20.47"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601686"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(95)00010-D"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/rpd\/ncl431"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR3182"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404073200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.10049"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/138620706777698481"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000310001632895"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg135"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050162"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4332"},{"key":"e_1_2_6_24_1","first-page":"5797","article-title":"Alpha\u2010particle\u2010induced p53 protein expression in a rat lung epithelial cell strain","volume":"54","author":"Hickman AW","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi224"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.2.2821"},{"key":"e_1_2_6_27_1","first-page":"1290","article-title":"Factors underlying the cell growth\u2010related bystander responses to alpha particles","volume":"60","author":"Iyer R","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_28_1","first-page":"164","article-title":"Radioresistance of rat glioma cell lines cultured as multicellular spheroids. 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