{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:56:09Z","timestamp":1791140169566,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,21]],"date-time":"2007-05-21T00:00:00Z","timestamp":1179705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The proteasome is the main protease for extralysosomal protein degradation in eukaryotic cells, and constitutes a sophisticated high molecular mass proteinase complex underlying a tightly coordinated expression and assembly of multiple subunits and subcomplexes. Here we show that continuous inhibition of proteasomal chymotrypsin\u2010like peptidase activity by the proteasome inhibitor bortezomib induces in human Namalwa Burkitt lymphoma cells increased de novo biogenesis of proteasomes accompanied by increased expression of the proteasome maturation protein POMP, increased expression of 19S\u201020S\u201019S proteasomes, and abrogation of expression of \u03b21i, \u03b22i and \u03b25i immunosubunits and PA28 in favor of increased expression of constitutive proteolytic \u03b21, \u03b22 and \u03b25 subunits and 19S regulatory complexes. These alterations of proteasome expression and subunit composition are accompanied by an increase in proteasomal caspase\u2010like, trypsin\u2010like and chymotrypsin\u2010like peptidase activities, not inhibitable by high doses of bortezomib. Cells harboring these proteasomal alterations display rapid proliferation and cell cycle progression, and acquire resistance to apoptosis induced by proteasome inhibitors, \u03b3\u2010irradiation and staurosporine. This acquired apoptosis resistance is accompanied by de novo expression of anti\u2010apoptotic Hsp27 protein and the loss of ability to accumulate and stabilize pro\u2010apoptotic p53 protein. Thus, increased expression, altered subunit composition and increased activity of proteasomes constitute a hitherto unknown adaptive and autoregulatory feedback mechanism to allow cells to survive the lethal challenge of proteasome inhibition and to establish a hyperproliferative and apoptosis\u2010resistant phenotype. J. Cell. Biochem. 103: 270\u2013283, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21405","type":"journal-article","created":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T05:16:00Z","timestamp":1179810960000},"page":"270-283","source":"Crossref","is-referenced-by-count":68,"title":["Increased expression and altered subunit composition of proteasomes induced by continuous proteasome inhibition establish apoptosis resistance and hyperproliferation of Burkitt lymphoma cells"],"prefix":"10.1002","volume":"103","author":[{"given":"Dominik","family":"Fuchs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Berges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Opelz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Daniel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cord","family":"Naujokat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80929-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35023595"},{"key":"e_1_2_6_4_1","first-page":"6174","article-title":"Blockade of Hsp27 overcomes Bortezomib\/proteasome inhibitor PS\u2010341 resistance in lymphoma cells","volume":"63","author":"Chauhan D","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_1","first-page":"239","article-title":"Role of p53 in cell cycle regulation and apoptosis following exposure to proteasome inhibitors","volume":"11","author":"Chen F","year":"2000","journal-title":"Cell Growth Differ"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000192261.02023.b8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209974"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4185"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/373438a0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.40.25637"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.14.2061"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35060076"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5404.978"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/33443"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00027.2001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81603-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.09.025"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020801"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00990970"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003139"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80341-X"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509043200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35056572"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404414"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(01)01241-X"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-003-0010-4"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/416763a"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02813.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.12893"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb04608.x"},{"key":"e_1_2_6_31_1","first-page":"2649","article-title":"In vivo ubiquitination and proteasome\u2010mediated degradation of p53","volume":"56","author":"Maki CG","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.42.26103"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301032200"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000022226.23741.37"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0609.2000.065004221.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.08.141"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(91)90198-O"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.02.106"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.16.5790-5802.2003"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403109"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.2.513"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.11.030"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.57.042905.122625"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-B"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-04-0311"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37158-3"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.19.14336"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.68.1.1015"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.180328897"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403551200"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3959"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109996200"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401253200"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208225"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.21405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.21405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T08:22:56Z","timestamp":1696926176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.21405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,21]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1002\/jcb.21405"],"URL":"https:\/\/doi.org\/10.1002\/jcb.21405","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,21]]}}}