{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:00:00Z","timestamp":1762297200274},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T00:00:00Z","timestamp":1180915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tumor suppressor p53 is an essential regulator in mammalian cellular responses to DNA damage including cell cycle arrest and apoptosis. Our study with Chinese hamster ovary CHO\u2010K1 cells indicates that when p53 expression and its transactivation capacity was inhibited by siRNA, UVC\u2010induced G2\/M arrest or apoptosis were unaffected as revealed by flow cyotmetric analyses and other measurements. However, inhibition of p53 rendered the cells slower to repair UV\u2010induced damages upon a plasmid as shown in host cell reactivation assay. Furthermore, the nuclear extract (NE) of p53 siRNA\u2010treated cells was inactive to excise the UV\u2010induced DNA adducts as analyzed by comet assay. Consistently, the immunodepletion of p53 also deprived the excision activity of the NE in the similar experiment. Thus, tumor suppressor p53 of CHO\u2010K1 cells may facilitate removal of UV\u2010induced DNA damages partly via its involvement in the repair mechanism. J. Cell. Biochem. 103: 528\u2013537, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21428","type":"journal-article","created":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T16:46:30Z","timestamp":1180975590000},"page":"528-537","source":"Crossref","is-referenced-by-count":17,"title":["Expression of tumor suppressor p53 facilitates DNA repair but not UV\u2010induced G2\/M arrest or apoptosis in Chinese hamster ovary CHO\u2010K1 cells"],"prefix":"10.1002","volume":"103","author":[{"given":"Yu\u2010Ching","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chu\u2010Bin","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Yu Chiang","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Li","family":"Liou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin\u2010Chang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,4]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0494"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202485699"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-200201000-00015"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/m79-154"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2003.10.017"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.44.28073"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206138"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80002-7"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.424"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.5.2147"},{"issue":"10","key":"e_1_2_5_12_1","first-page":"2001","article-title":"MDM2 protein confers the resistance of a human glioblastoma cell line to cisplatin\u2010induced apoptosis","volume":"10","author":"Kondo S","year":"1995","journal-title":"Oncogene"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.02.005"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20688"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1988.tb02785.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.04.032"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7801120"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006990200"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1546"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2004.64"},{"issue":"6","key":"e_1_2_5_21_1","first-page":"1053","article-title":"Involvement of the p53 tumor suppressor in repair of u.v.\u2010type DNA damage","volume":"10","author":"Smith ML","year":"1995","journal-title":"Oncogene"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204252"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/104454999315376"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-7864(03)00002-8"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.10.024"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01920109"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.4.914"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.21428","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.21428","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T19:00:43Z","timestamp":1696791643000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.21428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,4]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1002\/jcb.21428"],"URL":"https:\/\/doi.org\/10.1002\/jcb.21428","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,4]]}}}