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Metastatic tumor cells can produce platelet \u03b1IIb\u03b23 activators, such as ADP and thromboxane A<jats:sub>2<\/jats:sub> (TXA<jats:sub>2<\/jats:sub>). Inhibitors of platelet \u03b23 integrins decrease bone metastases in mice but are associated with significant bleeding. We examined the role of a novel soluble apyrase\/ADPase, APT102, and an inhibitor of TXA<jats:sub>2<\/jats:sub> synthesis, acetylsalicylic acid (aspirin or ASA), in mouse models of experimental bone metastases. We found that treatment with ASA and APT102 in combination (ASA\u2009+\u2009APT102), but not either drug alone, significantly decreased breast cancer and melanoma bone metastases in mice with fewer bleeding complications than observed with \u03b1IIb\u03b23 inhibition. ASA\u2009+\u2009APT102 diminished tumor cell induced platelet aggregation but did not directly alter tumor cell viability. Notably, APT102\u2009+\u2009ASA treatment did not affect initial tumor cell distribution and similar results were observed in \u03b23\u2212\/\u2212 mice. These results show that treatment with ASA\u2009+\u2009APT102 decreases bone metastases without significant bleeding complications. Anti\u2010platelet drugs such as ASA\u2009+\u2009APT102 could be valuable experimental tools for studying the role of platelet activation in metastasis as well as a therapeutic option for the prevention of bone metastases. J. Cell. Biochem. 104: 1311\u20131323, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21709","type":"journal-article","created":{"date-parts":[[2008,2,7]],"date-time":"2008-02-07T22:16:28Z","timestamp":1202422588000},"page":"1311-1323","source":"Crossref","is-referenced-by-count":58,"title":["APT102, a novel adpase, cooperates with aspirin to disrupt bone metastasis in mice"],"prefix":"10.1002","volume":"104","author":[{"given":"\u00d6zge","family":"Ulu\u00e7kan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C.","family":"Eagleton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree H.","family":"Floyd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela 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