{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:59:15Z","timestamp":1791284355372,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T00:00:00Z","timestamp":1216857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2008,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously demonstrated induction of <jats:italic>O<\/jats:italic>\u2010acetylated sialoglycoproteins on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). These molecules promote survival of lymphoblasts by preventing apoptosis. Although <jats:italic>O<\/jats:italic>\u2010acetylated sialoglycoproteins are over expressed, the status of <jats:italic>O<\/jats:italic>\u2010acetylation of gangliosides and their role in lymphoblasts survival remains to be explored in ALL patients. Here, we have observed enhanced levels of 9\u2010<jats:italic>O<\/jats:italic>\u2010acetylated GD3 (9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3) in the lymphoblasts of patients and leukaemic cell line versus disialoganglioside GD3 in comparison to the normal cells. Localization of GD3 and 9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3 on mitochondria of patient's lymphoblasts has been demonstrated by immuno\u2010electron microscopy. The exogenous administration of GD3\u2010induced apoptosis in lymphoblasts as evident from the nuclear fragmentation and sub G0\/G1 apoptotic peak. In contrast, 9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3 failed to induce such apoptosis. We further explored the mitochondria\u2010dependent pathway triggered during GD3\u2010induced apoptosis in lymphoblasts. GD3 caused a time\u2010dependent depolarization of mitochondrial membrane potential, release of cytochrome <jats:italic>c<\/jats:italic> and 7.4\u2010 and 8\u2010fold increased in caspase 9 and caspase 3 activity respectively. However, under identical conditions, an equimolar concentration of 9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3 failed to induce similar effects. Interestingly, 9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3 protected the lymphoblasts from GD3\u2010induced apoptosis when administered in equimolar concentrations simultaneously. In situ de\u2010<jats:italic>O<\/jats:italic>\u2010acetylation of 9\u2010<jats:italic>O<\/jats:italic>\u2010AcGD3 with sodium salicylate restores the GD3\u2010responsiveness to apoptotic signals. Although both GD3 and 9\u2010<jats:italic>O<\/jats:italic>\u2010acetyl GD3 localize to mitochondria, these two structurally related molecules may play different roles in ALL\u2010disease biology. Taken together, our results suggest that <jats:italic>O<\/jats:italic>\u2010acetylation of GD3, like that of <jats:italic>O<\/jats:italic>\u2010acetylated sialoglycoproteins, might be a general strategy adopted by leukaemic blasts towards survival in ALL. J. Cell. Biochem. 105: 724\u2013734, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.21867","type":"journal-article","created":{"date-parts":[[2008,7,24]],"date-time":"2008-07-24T19:50:43Z","timestamp":1216929043000},"page":"724-734","source":"Crossref","is-referenced-by-count":56,"title":["<i>O<\/i>\u2010acetylation of GD3 prevents its apoptotic effect and promotes survival of lymphoblasts in childhood acute lymphoblastic leukaemia"],"prefix":"10.1002","volume":"105","author":[{"given":"Kankana","family":"Mukherjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Chava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandan","family":"Mandal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sailendra 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