{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T10:45:27Z","timestamp":1755600327079},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,5,18]],"date-time":"2009-05-18T00:00:00Z","timestamp":1242604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>SVS I<\/jats:italic> was exclusively expressed in seminal vesicle in which the protein was immunolocalized primarily to the luminal epithelium of mucosal folds. The developmental profile of its mRNA expression was shown to be androgen\u2010dependent, manifesting a positive correlation with the animal's maturation. There are 43 glutamine and 43 lysine residues in one molecule of SVS I, which is one of the seven major monomer proteins tentatively assigned on reducing SDS\u2013PAGE during the resolution of mouse seminal vesicle secretion. Based on the fact that <jats:italic>SVS I<\/jats:italic>\u2010deduced protein sequence consists of 796 amino acid residues, we produced 7 recombinant polypeptide fragments including residues 1\u201378\/F1, residues 79\u2013259\/F2, residues 260\u2013405\/F3, residues 406\u2013500\/F4, residues 501\u2013650\/F5, residues 651\u2013715\/F6, and residues 716\u2013796\/F7, and measured the covalent incorporation of 5\u2010(biotinamido)pentylamine (BPNH<jats:sub>2<\/jats:sub>) or biotin\u2010TVQQEL (A25 peptide) to each of F1\u2010to\u2010F7 by type 4 transglutaminase (TG<jats:sub>4<\/jats:sub>) from the coagulating gland secretion. F2 was active to a greater extent than the other fragments during the BPNH<jats:sub>2<\/jats:sub>\u2010glutamine incorporation, and a relatively low extent of A25\u2010lysine cross link was observed with all of the seven fragments. The MS analysis of BPNH<jats:sub>2<\/jats:sub>\u2010F2 conjugate identified Q<jats:sup>232<\/jats:sup> and Q<jats:sup>254<\/jats:sup> as the two major TG<jats:sub>4<\/jats:sub> cross\u2010linking sites. This was substantiated by the result that much less BPNH<jats:sub>2<\/jats:sub> was cross\u2010linked to any one of the three F2 mutants, including Q232G and Q254G obtained from single\u2010site mutation, and Q232G\/Q254G from double\u2010site mutation. J. Cell. Biochem. 107: 899\u2013907, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22190","type":"journal-article","created":{"date-parts":[[2009,5,18]],"date-time":"2009-05-18T19:34:59Z","timestamp":1242675299000},"page":"899-907","source":"Crossref","is-referenced-by-count":12,"title":["Identification of the major TG<sub>4<\/sub> cross\u2010linking sites in the androgen\u2010dependent SVS I exclusively expressed in mouse seminal vesicle"],"prefix":"10.1002","volume":"107","author":[{"given":"Huan\u2010Chin","family":"Tseng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han\u2010Jia","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyh\u2010Bing","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.S. 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