{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T07:41:13Z","timestamp":1697010073353},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T00:00:00Z","timestamp":1245024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glycosylation is one of the most important post\u2010translational modifications. It is clear that the single step of \u03b2\u20101,4\u2010galactosylation is performed by a family of \u03b2\u20101,4\u2010galactosyltransferases (\u03b2\u20101,4\u2010GalTs), and that each member of this family may play a distinct role in different tissues and cells. In the present study, real\u2010time PCR revealed that the \u03b2\u20101,4\u2010GalT I mRNA reached peaks at 2 weeks after sciatic nerve crush and 3 days after sciatic nerve transection. Combined in situ hybridization for \u03b2\u20101,4\u2010GalT I mRNA and immunohistochemistry for S100 showed that \u03b2\u20101,4\u2010GalT I mRNAs were mainly located in Schwann cells after sciatic nerve injury. In conclusion, \u03b2\u20101,4\u2010GalT I might play important roles in Schwann cells during the regeneration and degeneration of the injured sciatic nerve. In other pathology, such as inflammation, we found that LPS administration affected \u03b2\u20101,4\u2010GalT I mRNA expression in sciatic nerve in a time\u2010 and dose\u2010dependent manner, and \u03b2\u20101,4\u2010GalT I mRNA is expressed mainly in Schwann cells. These results indicated that \u03b2\u20101,4\u2010GalT I plays an important role in the inflammation reaction induced by intraperitoneal injection of LPS. Similarly, we found that \u03b2\u20101,4\u2010GalT I in Schwann cells in vitro was affected in a time\u2010 and concentration\u2010dependent manner in response to LPS stimulation. All these results suggest that \u03b2\u20101,4\u2010GalT I play an important role in Schwann cells in vivo and vitro during pathology. In addition, \u03b2\u20101,4\u2010GalT I production was drastically suppressed by U0126 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (SAPK\/JNK inhibitor), which indicated that Schwann cells which regulated \u03b2\u20101,4\u2010GalT I expression after LPS stimulation were via ERK, SAPK\/JNK, and P38 MAP kinase signal pathways. J. Cell. Biochem. 108: 75\u201386, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22229","type":"journal-article","created":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T19:34:21Z","timestamp":1245094461000},"page":"75-86","source":"Crossref","is-referenced-by-count":6,"title":["Expression of \u03b2\u20101,4\u2010galactosyltransferase I in rat Schwann 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