{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:55:34Z","timestamp":1790978134642,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T00:00:00Z","timestamp":1249603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2009,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fas and FasL expression upregulation was found in human leukemia K562 cells upon exposure to <jats:italic>Naja naja atra<\/jats:italic> phospholipase A<jats:sub>2<\/jats:sub> (PLA<jats:sub>2<\/jats:sub>). PLA<jats:sub>2<\/jats:sub> treatment induced an increase in intracellular Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]i) and ROS generation levels, leading to activation of p38 MAPK and JNK. Suppression of both p38 MAPK and JNK abrogated Fas and FasL upregulation. Unlike PLA<jats:sub>2<\/jats:sub>, catalytically inactive PLA<jats:sub>2<\/jats:sub> treatment did not markedly increase Fas and FasL protein expression, and p38 MAPK activation was exclusively responsible for catalytically inactive PLA<jats:sub>2<\/jats:sub>\u2010induced increase in Fas and FasL protein expression. Knockdown of p38\u03b1 MAPK and JNK1 by siRNA proved that p38\u03b1 MAPK and JNK1 were involved in ATF\u20102 and c\u2010Jun phosphorylation, respectively. Compared with the p38\u03b1 MAPK\/ATF\u20102 pathway, the JNK1\/c\u2010Jun pathway played a crucial role in Fas\/FasL upregulation. Unlike arachidonic acid, lysophosphatidylcholine mimicked the PLA<jats:sub>2<\/jats:sub> action in inducing Fas\/FasL upregulation. Together with the previous finding that c\u2010Jun and ATF\u20102 are involved in transcriptional regulation of Fas and FasL, our data suggest that PLA<jats:sub>2<\/jats:sub> induces Fas and FasL upregulation through p38\u03b1 MAPK\/ATF\u20102 and JNK1\/c\u2010Jun pathways in K562 cells, and PLA<jats:sub>2<\/jats:sub> catalytic activity is involved in this action. J. Cell. Biochem. 108: 612\u2013620, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22293","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T06:20:38Z","timestamp":1253686838000},"page":"612-620","source":"Crossref","is-referenced-by-count":13,"title":["JNK1\/c\u2010Jun and p38\u03b1 MAPK\/ATF\u20102 pathways are responsible for upregulation of Fas\/FasL in human chronic myeloid leukemia K562 cells upon exposure to Taiwan cobra phospholipase A<sub>2<\/sub>"],"prefix":"10.1002","volume":"108","author":[{"given":"Ku\u2010Chung","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ling","family":"Chiou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long\u2010Sen","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,8,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1305"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022177"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21979"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgl119"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306220200"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800908784293640"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-1484"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.3.1741"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.6.1301"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199907153410306"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.9.5414"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.149"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402741"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80054-4"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/35037728"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8170-7"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6849"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/107327480501200203"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.062405.154007"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00220-1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-006-4689-y"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21713"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2008.12.004"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2007.12.003"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.1.186.bloodjournal851186"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10279"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2006.07.003"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4840"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2003.1.1"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.22293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.22293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T22:47:50Z","timestamp":1697582870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.22293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,7]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,10,15]]}},"alternative-id":["10.1002\/jcb.22293"],"URL":"https:\/\/doi.org\/10.1002\/jcb.22293","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,7]]}}}