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Extensive expansion of the cell cultures was performed and early and late passage cells (passages 4 and 9, respectively) were used to study signs of cellular aging, telomere length, telomerase activity, and cell\u2010cycle\u2010related gene expression. Our results showed that cellular aging was more prominent in bMSCs than in oMSCs, and that oMSCs had longer telomere length in late passages compared with bMSCs, although there was no significant difference in telomere lengths in the early passages in either cell type. Telomerase activity was detectable only in early passage oMSCs and not in bMSCs. In osteophyte tissues telomerase\u2010positive cells were found to be located perivascularly and were Stro\u20101 positive. Fifteen cell\u2010cycle regulator genes were investigated and only three genes (<jats:italic>APC<\/jats:italic>, <jats:italic>CCND2<\/jats:italic>, and <jats:italic>BMP2<\/jats:italic>) were differentially expressed between bMSC and oMSC. Our results indicate that oMSCs retain a level of telomerase activity in vitro, which may account for the relatively greater longevity of these cells, compared with bMSCs, by preventing replicative senescence. J. Cell. Biochem. 108: 839\u2013850, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22312","type":"journal-article","created":{"date-parts":[[2009,8,19]],"date-time":"2009-08-19T13:17:13Z","timestamp":1250687833000},"page":"839-850","source":"Crossref","is-referenced-by-count":15,"title":["Cellular senescence and longevity of osteophyte\u2010derived mesenchymal stem cells compared to patient\u2010matched bone marrow stromal 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