{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:09:35Z","timestamp":1783948175418,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T00:00:00Z","timestamp":1268092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2010,3,9]],"date-time":"2010-03-09T00:00:00Z","timestamp":1268092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Reference genes are generally employed in real\u2010time quantitative PCR (RT\u2010qPCR) experiments to normalize variability between different samples. The aim of this study was to identify and validate appropriate reference genes as internal controls for RT\u2010qPCR experiments in rubella virus (RV)\u2010infected Vero and MCF\u20107 cell lines using SYBR green fluorescence. The software programs geNorm and NormFinder and the \u0394\u0394C\n                    <jats:sub>t<\/jats:sub>\n                    calculation were used to determine the expression stability and thus reliability of nine suitable reference genes. HPRT1 and HUEL, and HUEL and TBP were identified to be most suitable for RT\u2010qPCR analysis of RV\u2010infected Vero and MCF\u20107 cells, respectively. These genes were used as normalizers for transcriptional activity of selected cellular genes. The results confirm previously published microarray and Northern blot data, particularly on the transcriptional activity of the cyclin\u2010dependent kinase inhibitor p21 and the nuclear body protein SP100. Furthermore, the mRNA level of the mitochondrial protein p32 is increased in RV\u2010infected cells. The effect on cellular gene transcription by RV\u2010infection seems to be cell line\u2010specific, but genes of central importance for viral life cycle appear to be altered to a similar degree. This study does not only provide an accurate and flexible tool for the quantitative analysis of gene expression patterns in RV\u2010infected cell lines. It also indicates, that the suitability of a reference gene as normalizer of RT\u2010qPCR data and the host\u2010cell response to RV\u2010infection are strictly cell\u2010line specific. J. Cell. Biochem. 110: 118\u2013128, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jcb.22518","type":"journal-article","created":{"date-parts":[[2010,3,10]],"date-time":"2010-03-10T01:34:12Z","timestamp":1268184852000},"page":"118-128","source":"Crossref","is-referenced-by-count":21,"title":["Validation and application of normalization factors for gene expression studies in rubella virus\u2010infected cell lines with quantitative real\u2010time PCR"],"prefix":"10.1002","volume":"110","author":[{"given":"S.","family":"Chey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Claus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.G.","family":"Liebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,3,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.08.003"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10807-10820.2005"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-43"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-350"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82035-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000898"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5259"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00140-9"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-9-49"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.9.765"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.41.25253"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364190"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02732-07"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.51.185"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229202. 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