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We have previously found that the mbr is a regulatory element which positively regulates BCL2 expression and this regulatory function was closely associated with SATB1, which binds to a 37\u2009bp mbr (37\u2009mbr) in the 3\u2032\u2010end of the mbr directly. However, the precise molecular mechanisms by which the mbr regulates gene expression are not fully understood. In this study, we purified Poly(ADP\u2010ribose) polymerase\u20101 (PARP\u20101) from the DNA\u2013protein complexes formed by 37\u2009mbr in Jurkat cells and demonstrated that PARP\u20101 participates in the 37\u2009mbr\u2013protein complex's formation in vitro and in vivo. Functional analysis showed that overexpression of PARP\u20101 decreases 37\u2009mbr regulatory function and BCL2 expression. Conversely, knockdown of PARP\u20101 with RNAi increases BCL2 expression. Taken together, the present findings indicate that PARP\u20101 is a component of BCL2 37\u2009mbr\u2013protein complexes, and PARP\u20101 is involved in the regulation of BCL2 expression. These findings are helpful in understanding the regulatory mechanisms of BCL2 expression. J. Cell. Biochem. 110: 1208\u20131218, 2010. Published 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22635","type":"journal-article","created":{"date-parts":[[2010,5,17]],"date-time":"2010-05-17T17:10:05Z","timestamp":1274116205000},"page":"1208-1218","source":"Crossref","is-referenced-by-count":9,"title":["Poly (ADP\u2010ribose) polymerase\u20101 binds to BCL2 major breakpoint region and regulates BCL2 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