{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:36:25Z","timestamp":1778600185364,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,11,29]],"date-time":"2010-11-29T00:00:00Z","timestamp":1290988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Eugenia punicifolia<\/jats:italic> known as \u201cpedra\u2010ume ca\u00e1\u201d is a shrub largely distributed in the Amazon region popularly used in decoctions or infusions as a natural therapeutic agent, which can interfere on cholinergic nicotinic neurotransmission. This work aimed to investigate a putative anti\u2010inflammatory effect of dichloromethane fraction of <jats:italic>E. punicifolia<\/jats:italic> extract (<jats:italic>Ep<\/jats:italic>\u2010CM) in the muscular lesion of <jats:italic>mdx<\/jats:italic> dystrophic mice, considering that activation of cholinergic mechanisms mitigates inflammation. A polymer containing the <jats:italic>Ep<\/jats:italic>\u2010CM was implanted in <jats:italic>mdx<\/jats:italic> gastrocnemius muscle before onset of myonecrosis for local slow and gradual release of bioactive compounds and mice sacrificed 7 days or 9 weeks after surgery. Comparing to control muscle, treatment did not alter choline acetyltransferase and acetylcholinesterase enzymatic activities, but decreased metaloproteases\u20109 and \u20102 activities and levels of tumor necrosis factor \u03b1 and NF\u03baB transcription factor. In addition, treatment also reduced levels of bioactive IL\u20101\u03b2 form and cleaved caspase\u20103, related to early events of cellular death and inflammatory activation and further increased myogenin expression without affecting collagen production which is associated with fibrosis. In vivo treatment of <jats:italic>mdx<\/jats:italic> dystrophic mice with <jats:italic>Ep<\/jats:italic>\u2010CM caused significant reduction of muscular inflammation and improved skeletal muscle regeneration without inducing fibrosis. J. Cell. Biochem. 111: 1652\u20131660, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22906","type":"journal-article","created":{"date-parts":[[2010,11,6]],"date-time":"2010-11-06T21:47:56Z","timestamp":1289080076000},"page":"1652-1660","source":"Crossref","is-referenced-by-count":7,"title":["Anti\u2010inflammatory activity of <i>Eugenia punicifolia<\/i> extract on muscular lesion of <i>mdx<\/i> dystrophic mice"],"prefix":"10.1002","volume":"111","author":[{"given":"Paulo Em\u00edlio Corr\u00eaa","family":"Leite","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kessiane Belshoff","family":"de 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