{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T17:55:28Z","timestamp":1718387728144},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T00:00:00Z","timestamp":1297728000000},"content-version":"vor","delay-in-days":45,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Burkitt lymphoma (BL) commonly exhibits Epstein\u2013Barr virus (EBV) positivity associated with latent chronic infection. Models of acute EBV infection have been associated with cellular resistance to apoptosis. However, the effect of latent long\u2010term EBV infection on apoptosis induced by drugs is not well defined. To determine this, we have studied the response of the Akata EBV+ cell line (type I latency) to etoposide, before and after downregulating EBV gene expression. We observed that downregulating EBV nuclear antigen\u20101 (EBNA\u20101) expression with siRNAs reverted cellular sensitivity to etoposide. In accordance with this finding, Akata EBV+ cells showed increased sensitivity to etoposide, when compared to the Akata EBV\u2212 cells. We also observed that Akata EBV+ cells presented increased apoptosis levels and decreased <jats:italic>Bcl\u2010xL<\/jats:italic> mRNA and protein levels, when compared to the Akata EBV\u2212 cells. In addition, Akata EBV+ cells contained less endoplasmic reticulum (ER) than EBV\u2212 cells. Finally, downregulation of EBV with EBNA\u20101 siRNAs caused an increase in the expression of Bcl\u2010xL indicating that EBV is responsible for the differences found between the Akata EBV+ and EBV\u2212 cell lines. J. Cell. Biochem. 112: 200\u2013210, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.22920","type":"journal-article","created":{"date-parts":[[2010,11,10]],"date-time":"2010-11-10T16:46:26Z","timestamp":1289407586000},"page":"200-210","source":"Crossref","is-referenced-by-count":5,"title":["EBV interferes with the sensitivity of Burkitt lymphoma Akata cells to etoposide"],"prefix":"10.1002","volume":"112","author":[{"given":"Raquel T.","family":"Lima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo","family":"Seca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S\u00e3o Jos\u00e9","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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