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Analgesic\u2010antitumor peptide (AGAP), one of scorpion toxic polypeptides, has been shown to have antitumor activity. Here, we show that recombinant AGAP (rAGAP) not only inhibits the proliferation of gliomas cell SHG\u201044 and rat glioma cell C6, but also suppresses the migration of SHG\u201044 cells during wound healing. To explain these phenomena, we find that rAGAP leads to cell cycle of SHG\u201044 arrested in G1 phase accompanied by suppressing G1 cell cycle regulatory proteins CDK2, CDK6, and p\u2010RB by means of the down\u2010regulated protein expression of p\u2010AKT. Meanwhile, rAGAP significantly decreases the production of NF\u2010\u03baB, BCL\u20102, p\u2010p38, p\u2010c\u2010Jun, and p\u2010Erk1\/2 and further suppresses the activation of VEGF and MMP\u20109 in SHG\u201044 cells. These findings suggest rAGAP inhibit proliferation and migration of SHG\u201044 cells by arresting cell cycle and interfering p\u2010AKT, NF\u2010\u03baB, BCL\u20102, and MAPK signaling pathways. J. Cell. Biochem. 112: 2424\u20132434, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.23166","type":"journal-article","created":{"date-parts":[[2011,5,2]],"date-time":"2011-05-02T07:57:07Z","timestamp":1304323027000},"page":"2424-2434","source":"Crossref","is-referenced-by-count":53,"title":["Analgesic\u2010antitumor peptide inhibits proliferation and migration of SHG\u201044 human malignant glioma 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