{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:09:56Z","timestamp":1759176596518},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T00:00:00Z","timestamp":1327017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pathological bone destruction (osteolysis) is a hallmark of many bone diseases including tumor metastasis to bone, locally osteolytic giant cell tumor (GCT) of bone, and Paget's disease. Paclitaxel is frequently prescribed in the treatment of several malignant tumors where it has been shown to exert beneficial effects on bone lesions. However, the mechanism(s) through which paclitaxel regulates osteoclast formation and function remain ill defined. In the present study, we demonstrate that paclitaxel dose\u2010dependently inhibits receptor activator of nuclear factor\u2010kappa B ligand (RANKL)\u2010induced osteoclastogenesis in both RAW264.7 cells and mouse bone marrow macrophage (BMM) systems. In addition, paclitaxel treatment reduces the bone resorptive activity of human osteoclasts derived from GCT of bone, and attenuates lipopolysaccharide (LPS)\u2010induced osteolysis in a mouse calvarial model. Complementary cellular and biochemical analyses revealed that paclitaxel induces mitotic arrest of osteoclastic precursor cells. Furthermore, luciferase reporter gene assays and western blot analysis indicate that paclitaxel modulates key RANKL\u2010induced activation pathways that are essential to osteoclast formation including NF\u2010\u03baB and ERK. Collectively, our findings demonstrate a role for paclitaxel in the regulation of osteoclast formation and function and uncover potential mechanism(s) through which paclitaxel alleviates pathological osteolysis. J. Cell. Biochem. 113: 946\u2013955, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.23423","type":"journal-article","created":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T13:15:22Z","timestamp":1319721322000},"page":"946-955","source":"Crossref","is-referenced-by-count":17,"title":["Paclitaxel inhibits osteoclast formation and bone resorption via influencing mitotic cell cycle arrest and RANKL\u2010induced activation of NF\u2010\u03baB and ERK"],"prefix":"10.1002","volume":"113","author":[{"given":"Estabelle S. M.","family":"Ang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan J.","family":"Pavlos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shek Man","family":"Chim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao Tian","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin M.","family":"Scaife","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James H.","family":"Steer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming H.","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009033481967"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22800"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.07.046"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21076"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204062"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207233"},{"key":"e_1_2_6_8_1","first-page":"27","article-title":"Paclitaxel\u2010induced apoptosis may occur without a prior G2\/M\u2010phase arrest","volume":"24","author":"Dziadyk JM","year":"2004","journal-title":"Anticancer Res"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00301951"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19971015)80:8 <1646::AID-CNCR14>3.0.CO;2-#"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208284200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64942-5"},{"key":"e_1_2_6_13_1","first-page":"265","article-title":"Activation of NF\u2010kappa B in murine macrophages by taxol","volume":"14","author":"Hwang S","year":"1995","journal-title":"Cancer Biochem Biophys"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1317"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3915-3922.2002"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0958"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2003.18.7.1198"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.12.5253-5269.2005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1128\/iai.64.3.878-884.1996","article-title":"Paclitaxel (Taxol)\u2010induced NF\u2010kappaB translocation in murine macrophages","volume":"64","author":"Perera PY","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2003.13357.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685559"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0009"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4731"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060834"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(95)01367-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2003.18.12.2159"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.7.1200"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10477"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2178"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2008.11.007"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050960"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.040919"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050324"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.23423","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.23423","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T09:11:20Z","timestamp":1696929080000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.23423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,20]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/jcb.23423"],"URL":"https:\/\/doi.org\/10.1002\/jcb.23423","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,20]]}}}