{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T11:48:40Z","timestamp":1789559320872,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T00:00:00Z","timestamp":1331769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>RANKL\u2010stimulation of osteoclast precursors results in up\u2010regulation of genes involved in the process of differentiation and activation. In this report we describe the expression and functional characterization of <jats:italic>Sorting Nexin 10<\/jats:italic> (<jats:italic>snx10<\/jats:italic>). Snx10 belongs to the sorting nexin (SNX) family, a diverse group of proteins with a common feature: the PX domain, which is involved in membrane trafficking and cargo sorting in endosomes. <jats:italic>Snx10<\/jats:italic> is strongly up\u2010regulated during RANKL\u2010induced osteoclast differentiation in vitro and expressed in osteoclasts in vivo. qPCR analysis confirmed a significant increase in the expression of <jats:italic>snx10<\/jats:italic> in in vitro\u2010derived osteoclasts, as well as in femur and calvaria. Immunohistochemical analysis of mouse embryo sections showed expression in long bone, calvariae, and developing teeth. The expression was limited to cells that also expressed TRAP, demonstrating osteoclastic localization. Confocal immunofluorescence and subcellular fractionation analysis revealed Snx10 localization in the nucleus and in the endoplasmic reticulum (ER). To study a possible role for <jats:italic>snx10<\/jats:italic> in osteoclast differentiation and function we silenced <jats:italic>snx10<\/jats:italic> expression and found that <jats:italic>snx10<\/jats:italic> silencing inhibited RANKL\u2010induced osteoclast formation and osteoclast resorption on hydroxyapatite. Silencing also inhibited TRAP secretion. Taken together, these results confirm that <jats:italic>snx10<\/jats:italic> is expressed in osteoclasts and is required for osteoclast differentiation and activity in vitro. Since inhibition of vesicular trafficking is essential for osteoclast formation and activity and SNX10 is involved in intracellular vesicular trafficking, these studies may identify a new candidate gene involved in the development of human bone diseases including osteoporosis. J. Cell. Biochem. 113: 1608\u20131615, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24029","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T08:42:31Z","timestamp":1323938551000},"page":"1608-1615","source":"Crossref","is-referenced-by-count":48,"title":["SNX10 is required for osteoclast formation and resorption activity"],"prefix":"10.1002","volume":"113","author":[{"given":"C.H.","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. R.","family":"Morse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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