{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T12:08:01Z","timestamp":1747656481413},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":6623,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biochemical evidence is presented for the autophagic destruction of liver mitochondria in the influenza B virus model of Reye's syndrome in mice. Separation of lysosomes and autophagic vacuoles from mitochondria was accomplished by prior treatment of the mice with Triton WR\u20101339, resulting in uptake of detergent by these organelles (tritosomes), reducing their densities. The organelles were banded in a discontinuous sucrose gradient. Total protein in the heavy tritosomal fraction increased from 1\u20132% in controls to 7\u20138% in virus\u2010treated animals. Ornithine carbamoyl transferase (OCTase), a mitochondrial marker, increased from 2\u20133% (controls) to 11\u201315% (virus\u2010treated), and glucose\u20106\u2010phosphatase, a marker for endoplasmic reticulum, increased from 1\u20132% (controls) to 8\u201310% (virus\u2010treated). \u03b2\u2010Galactosidase, a soluble enzyme in the lysosome, and OCTase also increase in the cell extract fraction following virus treatment, indicating that there was turnover of heavy lysosomal contents.<\/jats:p>","DOI":"10.1002\/jcb.240310404","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T14:55:03Z","timestamp":1104418503000},"page":"271-275","source":"Crossref","is-referenced-by-count":15,"title":["Liver autophagy in the influenza B virus model of Reye's syndrome in mice"],"prefix":"10.1002","volume":"31","author":[{"given":"Beulah M.","family":"Woodfin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry E.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"140","volume":"48","author":"Davis LE","year":"1983","journal-title":"Lab Invest"},{"key":"e_1_2_1_3_2","first-page":"1026","volume":"23","author":"Swift H","year":"1964","journal-title":"Fed Proc"},{"key":"e_1_2_1_4_2","first-page":"345","volume-title":"Lysosomes in Biology and Pathology II","author":"Ericsson JL","year":"1969"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(81)90037-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197112092852402"},{"key":"e_1_2_1_7_2","first-page":"117","volume-title":"Reye's Syndrome","author":"Partin JC","year":"1975"},{"key":"e_1_2_1_8_2","first-page":"89","volume":"45","author":"Hug G","year":"1981","journal-title":"Lab Invest"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.37.2.482"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2944(82)90060-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90500-8"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0600604"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60149a611"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240310404","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240310404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T22:46:32Z","timestamp":1697928392000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240310404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/jcb.240310404"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240310404","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}