{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:15:52Z","timestamp":1791288952296,"version":"4.3.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":6623,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human plasma serine proteinase inhibitors (serpins) gradually lost activity when incubated with catalytic amounts of snake venom or bacterial metalloproteinases. Electrophoretic analyses indicated that antithrombin III, C<jats:styled-content>1<\/jats:styled-content>\u2010inhibitor, and \u03b1<jats:sub>2<\/jats:sub>\u2010antiplasmin had been converted by limited proteolysis into modified species which retained inhibitory activity. Further proteolytic attack resulted in the formation of inactivated inhibitors; \u03b1<jats:sub>1<\/jats:sub>\u2010proteinase inhibitor (\u03b1<jats:sub>1<\/jats:sub>\u2010antitrypsin) and \u03b1<jats:sub>1<\/jats:sub>\u2010antichymotrypsin were also enzymatically inactivated, but active intermediates were not detected. Sequence analyses indicated that the initial, noninactivating cleavage occurred in the amino\u2010terminal region of the inhibitors. Inactivation resulted in all cases from the limited proteolysis of a single bond near, but not at, the reactive site bond in the carboxy\u2010terminal region of the inhibitors. The results indicate that the serpins have two regions which are susceptible to limited proteolysis\u2014one near the amino\u2010terminal end and another in the exposed reactive site loop of the inhibitor.<\/jats:p>","DOI":"10.1002\/jcb.240320106","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T14:55:09Z","timestamp":1104418509000},"page":"51-58","source":"Crossref","is-referenced-by-count":44,"title":["Inactivation of human plasma serine proteinase inhibitors (serpins) by limited proteolysis of the reactive site loop with snake venom and bacterial metalloproteinases"],"prefix":"10.1002","volume":"32","author":[{"given":"Lawrence F.","family":"Kress","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91039-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34403-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(84)90057-8"},{"key":"e_1_2_1_5_2","first-page":"159","volume":"30","author":"Kress LF","year":"1983","journal-title":"Acta Biochim Polon"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/317730a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(79)80026-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89572-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)80058-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(83)90039-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90207-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50597-4"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00529a017"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34475-7"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(82)90155-8"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a134671"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(79)80532-3"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/312014a0"},{"key":"e_1_2_1_19_2","first-page":"431","volume":"778","author":"Kress LF","year":"1984","journal-title":"Comp Biochem Physiol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365517209102760"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.26.11.852"},{"key":"e_1_2_1_22_2","first-page":"81","volume":"122","author":"Stockley RA","year":"1980","journal-title":"Am Rev Resp Dis"},{"key":"e_1_2_1_23_2","first-page":"1232","volume":"42","author":"Salvesen G","year":"1983","journal-title":"Fed Proc"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/oxfordjournals.jbchem.a131900","volume":"83","author":"Morii M","year":"1978","journal-title":"J Biochem"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)90325-X"},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1128\/iai.24.1.188-193.1979","volume":"24","author":"Morihara K","year":"1979","journal-title":"Infect Immunol"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"9849","DOI":"10.1016\/S0021-9258(18)34149-8","volume":"257","author":"Brower MS","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"3931","DOI":"10.1016\/S0021-9258(19)85615-6","volume":"255","author":"Beany K","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(67)80055-X"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/298329a0"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00290a019"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240320106","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240320106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T22:52:31Z","timestamp":1697928751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240320106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/jcb.240320106"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240320106","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}