{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T12:21:12Z","timestamp":1759580472049},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5862,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1988,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytogenetic patterns from primary short\u2010term culture of breast cancer, renal carcinoma, and tumors of the central nervous system are presented to illustrate the range of karyotypic diversity of human solid tumors as well as their biologic differences in culture systems that support their growth. These studies have illustrated several major issues. (1) Results vary with the tissue of origin: primary cultures from breast are almost uniformly diploid, while renal tumors are near\u2010diploid, mosaic, and show clonal aberrations; and CNS tumors are heterogeneous: some diploid, some near\u2010diploid and some highly aneuploid. (2) Results after short\u2010term culture are selective, representing subpopulations from the heterogeneous cells that are detected on direct analysis of fresh tumors by cytogenetics or flow cytometry (FCM). It is not yet clear whether prognosis depends on the dominant population of the primary tumor or alternatively should be influenced by detection of small aneuploid subpopulations. (3) Evidence from all three tumor types supports the interpretation that cytogenetically normal diploid cells constitute part of some tumor populations, and may be better adapted to routine growth in culture than aneuploid subpopulations from the same primary tumors. These cells may also compose a major portion of the viable population of tumors in vivo and, therefore, could represent a useful model for studies of tumorigenesis and therapeutic regimens.<\/jats:p>","DOI":"10.1002\/jcb.240360206","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:40:12Z","timestamp":1104421212000},"page":"147-156","source":"Crossref","is-referenced-by-count":15,"title":["Cytogenetic diversity in primary human tumors"],"prefix":"10.1002","volume":"36","author":[{"given":"Sandra R.","family":"Wolman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia M.","family":"Camuto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Ann","family":"Perle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"6019","volume":"46","author":"Croce CM","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_3_2","first-page":"2223","volume":"43","author":"Seibert K","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90052-9"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(87)90082-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(85)90077-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","unstructured":"WolmanSR: In Medina D Kidwell W Heppner G: Anderson E (eds): \u201cCellular and Molecular Biology of Experimental Mammary Cancer.\u201d New York: Plenum Press 1987(in press).","DOI":"10.1007\/978-1-4613-0943-7"},{"key":"e_1_2_1_8_2","first-page":"4637","volume":"41","author":"Smith HS","year":"1981","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(84)90009-X"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.6.1805"},{"key":"e_1_2_1_11_2","first-page":"75","volume-title":"Breast Cancer: On the Frontiers of Discovery","author":"Smith HS","year":"1986"},{"key":"e_1_2_1_12_2","first-page":"1187","volume":"77","author":"Teyssier JR","year":"1986","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_1_13_2","first-page":"2139","volume":"46","author":"Yoshida MA","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(86)90145-7"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197909133011107"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90118-3"},{"key":"e_1_2_1_17_2","first-page":"4","volume":"13","author":"Shapiro JR","year":"1986","journal-title":"Semin Oncol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(86)90172-X"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-580450-9.50018-8"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240360206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240360206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T14:26:03Z","timestamp":1697898363000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240360206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,2]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,2]]}},"alternative-id":["10.1002\/jcb.240360206"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240360206","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,2]]}}}