{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:45:40Z","timestamp":1790970340962,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5558,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1988,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously identified by chemical cross\u2010linking a cell surface protein in Swiss 3T3 cells of apparent Mr 75,000\u201385,000, which may represent a major component of the receptor for peptides of the bombesin family in these cells [1]. Because bombesin\u2010like peptides may interact with other cell surface molecules, it was important to establish the correlation between receptor binding and functions of this complex and further characterize the Mr 75,000\u201385,000 cross\u2010linked protein. Detailed time courses carried out at different temperatures demonstrated that the Mr 75,000\u201385,000 affinity\u2010labelled band was the earliest cross\u2010linked complex detected in Swiss 3T3 cells incubated with <jats:sup>125<\/jats:sup>I\u2010labelled gastrin\u2010releasing peptide (<jats:sup>125<\/jats:sup>I\u2010GRP). Furthermore, the ability of various nonradioactive bombesin agonists and antagonists to block the formation of the Mr 75,000\u201385,000 cross\u2010linked complex correlated extremely well (r = 0.994) with the relative capacity of these peptides to inhibit <jats:sup>125<\/jats:sup>I\u2010GRP specific binding. Pretreatment with unlabelled GRP for up to 6 h caused only a slight decrease in both specific <jats:sup>125<\/jats:sup>I\u2010GRP binding and the affinity labelling of the Mr 75,000\u201385,000 protein. We also show that the cross\u2010linked complex is a glycoprotein. First, solubilized affinity labelled Mr 75,000\u201385,000 complex applied to wheat germ lectin\u2010sepharose columns was cluted by addition of 0.3 M N\u2010acetyl\u2010D\u2010glucosamine. Second, treatment with endo\u2010\u03b2\u2010N\u2010actylglucosaminidase F reduced the apparent molecular weight of the affinity\u2010labelled band from 75,000\u201385,000 to 43,000, indicating the presence of N\u2010linked oligosaccharide groups.<\/jats:p>","DOI":"10.1002\/jcb.240380403","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:41:36Z","timestamp":1104421296000},"page":"237-249","source":"Crossref","is-referenced-by-count":25,"title":["Characterization of a bombesin receptor on Swiss mouse 3T3 cells by affinity cross\u2010linking"],"prefix":"10.1002","volume":"38","author":[{"given":"James","family":"Sinnett\u2010Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Zachary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Rozengurt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90483-0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.10.2936"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02145873"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)91614-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)90814-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91611-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90471-1"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.22.7616"},{"key":"e_1_2_1_10_2","first-page":"729","volume":"4","author":"Zachary I","year":"1985","journal-title":"Cancer Surv"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3018928"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90513-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)75907-9"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.16.5768"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.6.2223"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.6.2211"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04584.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91101-0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)90424-8"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3465038"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90226-0"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041310211"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90120-0"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6272398"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-53-6-1310"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.7.2379"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/316823a0"},{"key":"e_1_2_1_28_2","first-page":"821","volume":"47","author":"Carney DN","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_29_2","first-page":"3947","volume":"262","author":"Zachary I","year":"1987","journal-title":"J Biol Client"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.15.4540"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.2.299"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/287607a0"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_34_2","unstructured":"WollPJ RozengurtE:Proc Natl Acad Sci USA(in press) 1988."},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02495.x"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)60946-9"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.000321"},{"key":"e_1_2_1_38_2","doi-asserted-by":"crossref","first-page":"4897","DOI":"10.1016\/S0021-9258(19)42406-X","volume":"249","author":"Koide N","year":"1974","journal-title":"J Biol Chem"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.71.1.159"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2644"},{"key":"e_1_2_1_41_2","doi-asserted-by":"crossref","first-page":"11489","DOI":"10.1016\/S0021-9258(18)33787-6","volume":"257","author":"Knipp MN","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_42_2","doi-asserted-by":"crossref","first-page":"5161","DOI":"10.1016\/S0021-9258(18)34650-7","volume":"257","author":"Bowen\u2010Pope DF","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34708-2"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44679-5"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.1048"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2384"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.01.110185.000245"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/329836a0"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.003151"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90551-1"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.12.4641"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240380403","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240380403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T04:03:58Z","timestamp":1697947438000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240380403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,12]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,12]]}},"alternative-id":["10.1002\/jcb.240380403"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240380403","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,12]]}}}