{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T15:14:35Z","timestamp":1760368475188},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5376,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phospholipase A<jats:sub>2<\/jats:sub> (PLA<jats:sub>2<\/jats:sub>) is a key component of the inflammatory process because of its role in the generation of eicosanoids and platelet\u2010activating factor (PAF). Manipulation of PLA<jats:sub>2<\/jats:sub> activity offers a novel therapeutic approach for the development of antiinflammatory agents; however, there is a need for a suitable in vivo model. Injection of 1 \u03bcg of snake venom PLA<jats:sub>2<\/jats:sub> (<jats:italic>A. piscivorus piscivorus<\/jats:italic>, D\u201049) into the mouse hind footpad produced a significant three\u2010 to four\u2010fold rise in paw edema within 10 min, compared to the saline control. Edema formation depended on enzyme concentration and appeared specific for PLA<jats:sub>2<\/jats:sub> since edema was negated by enzyme pretreatment with p\u2010bromophenacyl bromide, a nonspecific PLA<jats:sub>2<\/jats:sub> inhibitor. Moreover, injection of a protein such as bovine serum albumin did not result in significant edema. Coinjection of phenidone (lipoxygenase inhibitor, 50 \u03bcg), indomethacin (cyclooxygenase inhibitor, 50 \u03bcg), cyproheptadine (antihistamine\/antiserotonin, 50 \u03bcg), aristolochic acid (putative PLA<jats:sub>2<\/jats:sub> inhibitor, 100 \u03bcg), or kadsurenone (PAF antagonist, 50\u03bcg) with PLA<jats:sub>2<\/jats:sub> (1 \u03bcg\/paw) resulted in partial reduction (44.5, 34.2, 54.7, 64, and 50% inhibition, respectively) of edema formation. Oral administration of cyproheptadine (10 mg\/kg), indomethacin (10 mg\/kg), BW 755c (100 mg\/kg), or dexamethasone (1 mg\/kg) 1\u20133 h before challenge also decreased PLA<jats:sub>2<\/jats:sub>\u2010induced edema (63.0, 30.1, 47.8, or 62.5% inhibition, respectively). The data suggest that mouse paw edema resulting from PLA<jats:sub>2<\/jats:sub> injection is a multicomponent event, influenced by both autacoids and lipid mediators of inflammation.<\/jats:p>","DOI":"10.1002\/jcb.240400203","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:54:16Z","timestamp":1104422056000},"page":"147-155","source":"Crossref","is-referenced-by-count":23,"title":["Preliminary studies on phospholipase A<sub>2<\/sub>\u2010induced mouse paw edema as a model to evaluate antiinflammatory agents"],"prefix":"10.1002","volume":"40","author":[{"given":"L. A.","family":"Marshall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Y.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Calhoun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. P.","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(85)90640-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1983.tb11578.x"},{"key":"e_1_2_1_4_2","first-page":"288","volume":"115","author":"Maksen J","year":"1984","journal-title":"Am J Pathol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-1-349-81345-2_8","volume-title":"Mechanism of Steroid Action","author":"Flower RJ","year":"1981"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01966499"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(87)90286-8"},{"key":"e_1_2_1_8_2","first-page":"1","volume-title":"Advances in Inflammation Research","author":"Flower RJ","year":"1984"},{"key":"e_1_2_1_9_2","first-page":"854","volume":"46","author":"Marshall LA","year":"1987","journal-title":"Fed Proc"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/oxfordjournals.bmb.a071826","volume":"39","author":"Williams TJ","year":"1973","journal-title":"Br Med Bull"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(81)90149-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1985.tb05059.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/292455a0"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240400203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240400203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T20:11:53Z","timestamp":1698005513000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240400203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1002\/jcb.240400203"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240400203","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]}}}