{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T01:57:25Z","timestamp":1722218245516},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5315,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1989,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Raji\u2010HN2 is a B cell lymphoma (Burkitt lymphoma) line that was made resistant to nitrogen mustard. The drug\u2010resistant phenotype was accompanied by changes in gene expression. The expression of four unrelated genes was examined by Northern blot analysis. Raji\u2010HN2 cells were found to contain about twice the number of actin mRNA found in Raji cells. Both cell lines were found to contain equivalent amounts of \u03b22\u2010microglobulin, c\u2010myc oncogene, and immunoglobulin C\u03bc mRNAs. The C\u03bc mRNA was, however, larger in size in Raji\u2010HN2 cells. Alterations in actin and C\u03bc mRNAs in Raji\u2010HN2 cells were not due to gene amplification or rearrangement because Southern blot analysis revealed no changes in the genomic organization of these genes. The increased actin mRNA content was correlated with an increased actin content of Raji\u2010HN2 cells. The F\u2010actin (stained with 7\u2010nitrobenz\u20102\u2010oxa\u20101,3\u2010diazolylphallacidin) content of single cells was quantitated in a meridian interactive laser cytometer. Raji\u2010HN2 cells contained about twice the amount of F\u2010actin present in the parental Raji cells. Similar results were obtained when large populations, 10<jats:sup>6<\/jats:sup> cells each, were examined in a flow cytometer.<\/jats:p>","DOI":"10.1002\/jcb.240400402","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:21:48Z","timestamp":1104420108000},"page":"407-415","source":"Crossref","is-referenced-by-count":2,"title":["Altered actin and immunoglobulin C\u03bc expression in nitrogen mustard\u2010resistant human Burkitt lymphoma cells"],"prefix":"10.1002","volume":"40","author":[{"given":"K. 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