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All four B cell lines, although differing in their responsiveness to IFN\u2010\u03b3, were found to express high\u2010affinity receptors (1,000\u201311,000 receptors\/cell). Normal unactivated B lymphocytes were also found to express constitutively high\u2010affinity receptors, approximately 1,400 receptors per cell with an estimated affinity of 295 pM. Activation of the normal B cells in vitro with the polyclonal B cell activator, <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan strain I (SAC), resulted in a slight decline in receptor number and a more pronounced fall in receptor density. One of the B cell lines and unactivated normal B cells were shown to internalize labeled IFN\u2010\u03b3 rapidly. Chemical cross\u2010linking of <jats:sup>32<\/jats:sup>P\u2010IFN\u2010\u03b3 to the CB B cell line and to freshly isolated B lymphocytes revealed one major cross\u2010linked receptor\u2010ligand complex which had an estimated molecular weight of approximately 110 kilodaltons. This complex corresponded to a 93 kD receptor cross\u2010linked to recombinant IFN\u2010\u03b3. Our data indicate that normal B lymphocytes constitutively express an approximately 93 kD IFN\u2010\u03b3 receptor which is similar to the receptor present on Epstein\u2010Barr virus\u2010transformed B cell lines.<\/jats:p>","DOI":"10.1002\/jcb.240400403","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:21:48Z","timestamp":1104420108000},"page":"417-430","source":"Crossref","is-referenced-by-count":3,"title":["Demonstration and partial characterization of the interferon\u2010gamma receptor on human B lymphocytes"],"prefix":"10.1002","volume":"40","author":[{"given":"Toshimasa","family":"Nakagawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gita 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