{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:25:38Z","timestamp":1757701538254},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5072,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A homogeneous preparation of a urinary glycoprotein has been isolated from urine of patients with malignant melanoma and advanced adenocarcinomas of colon and lung. This molecule, Mr 30 kDa, is homologous to EDC1, a proteinase inhibitor antigenically related to plasma inter\u2010\u03b1\u2010trypsin inhibitor (IATI) originally isolated from the urine of a leukemic patient, E.D. The newly isolated EDC1 inhibits cellular proliferation of a Burkitt's lymphoma cell line, Raji, growing in serum\u2010free medium supplemented with insulin, transferrin, selenium, and linoleic acid. This concentration\u2010dependent inhibitory effect was monitored in terms of change in cell number and <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation. The growth of cells treated with \u223c3.3 pmol EDCl\/ml was 50% that of the control group by both assays. EDC1 was not cytotoxic to the cells because the EDC1\u2010treated cells excluded trypan blue and resumed normal growth after removal of EDC1. In addition, EDC1 treatment of Raji cells prelabeled with <jats:sup>3<\/jats:sup>H\u2010labeled DNA did not release more radioactivity into the conditioned medium than the untreated labeled cells. EDC1 did not affect the growth of Hs2B2, a B\u2010lymphoblast cell line, and Hs294T, a human malignant melanoma cell line. Equimolar and larger quantities of other proteinase inhibitors with inhibitory profiles similar to that of EDC1 (\u03b1\u20101 proteinase inhibitor, soybean trypsin inhibitor, lima bean trypsin inhibitor, and turkey ovomucoid) did not affect the growth of Raji cells. Raji cells have an absolute requirement of transferrin as a nutrient and require insulin to modulate the expression of transferrin receptors. The cells also synthesize interleukin\u2010I as an autocrine growth stimulator. EDC1 did not form a detectable complex with transferrin, insulin, or any autocrine factor synthesized by the cells.<\/jats:p>","DOI":"10.1002\/jcb.240420404","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:25:36Z","timestamp":1104420336000},"page":"207-217","source":"Crossref","is-referenced-by-count":11,"title":["Plasma inter\u2010\u03b1\u2010trypsin inhibitor\u2010related urinary glycoprotein EDC 1 inhibits the growth of a Burkitt's lymphoma cell line"],"prefix":"10.1002","volume":"42","author":[{"given":"Rajender K.","family":"Chawla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David 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