{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:25:45Z","timestamp":1784042745909,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":4280,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prognostic significance of the epidermal growth factor receptor status (EGF\u2010R\u2010status) for certain human tumors requires the development of antibodies useful for clinical application. We used purified receptor preparations to generate monoclonal antibodies immunoreactive with the EGF\u2010R purified from placenta membranes and A431 tumors. Four of the hybridomas contained antibodies (R2, R3, R5, and R9) which recognized both antigens. Antibody R3 was shown to display the following properties: it binds with a K<jats:sub>D<\/jats:sub> value of about 10<jats:sup>\u22129<\/jats:sup>\u201310<jats:sup>\u221210<\/jats:sup> M to the receptor, a half maximal inhibition of EGF\u2010binding is achieved at 5 \u00d7 10<jats:sup>\u22128<\/jats:sup> M, and in Western blots of cell membranes R3 specifically detects the EGF\u2010R at 0.1 \u03bc\/ml. R3 inhibits EGF\u2010dependent clonogenic growth of NRK cells and completely blocks EGF stimulated autophosphorylation of the receptor. Moreover, R3 also detects EGF\u2010R in paraffin\u2010embedded tissue sections taken from human salivary gland, term placenta, and adult skin and mammary carcinomas. Thus, R3 can be used in retrospective diagnostic clinical studies and might help to develop new immunotherapeutic intervention.<\/jats:p>","DOI":"10.1002\/jcb.240490208","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T20:57:25Z","timestamp":1117313845000},"page":"157-165","source":"Crossref","is-referenced-by-count":41,"title":["A new monoclonal antibody for detection of EGF\u2010receptors in western blots and paraffin\u2010embedded tissue sections"],"prefix":"10.1002","volume":"49","author":[{"given":"A.","family":"Fernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Spitzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.\u2010D.","family":"Boehmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Eckert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Zschiesche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Grosse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a072464"},{"key":"e_1_2_1_3_2","first-page":"285","article-title":"Expression of epidermal growth factor receptors on human cervical, ovarian, and vulval carcinomas","volume":"46","author":"Gullick WJ","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1989.113"},{"key":"e_1_2_1_5_2","first-page":"2104","article-title":"Expression of growth factor and epidermal growth factor receptor encoded transcripts in human gastric tissues","volume":"49","author":"Bennett C","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19900401)65:7<1619::AID-CNCR2820650728>3.0.CO;2-Q"},{"key":"e_1_2_1_7_2","first-page":"1398","article-title":"Epidermal growth factor receptor status as a predictor of early recurrence and death from breast cancer","volume":"1","author":"Sainsbury JRC","year":"1987","journal-title":"Lancet"},{"key":"e_1_2_1_8_2","first-page":"173","article-title":"Receptors for epidermal growth factor and estrogen as predictors of relapse in patients with mammary carcinomas","volume":"8","author":"Rios MA","year":"1988","journal-title":"Anticancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90856-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910390302"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01805739"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806484"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(88)80153-5"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000226840"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1991.130"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.38.11.1225"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"7609","DOI":"10.1016\/S0021-9258(19)44568-7","article-title":"Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization","volume":"247","author":"Savage RC","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/194495a0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.1.1"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/276269a0"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.20.6237"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0021-9258(18)89733-2","article-title":"Purification of an active EGF receptor kinase with monoclonal antireceptor antibodies","volume":"260","author":"Yarden Y","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_23_2","first-page":"27.1","volume-title":"Immuno\u2010chemistry","author":"Nakamura RM","year":"1986"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)91822-4"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1016\/S0021-9258(18)89586-2","article-title":"Amiloride directly inhibits growth factor receptor tyrosine kinase activity","volume":"260","author":"Davies RJ","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90281-5"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1989.7.8.1120"},{"key":"e_1_2_1_28_2","first-page":"642","article-title":"A Mr 43.000 epidermal growth factor\u2010related protein purified from the urine of breast cancer patients","volume":"50","author":"Eckert K","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(88)90068-5"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240200207"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.5.1337"},{"key":"e_1_2_1_32_2","first-page":"1550","article-title":"Characterization of murine monoclonal antibodies reactive to either the human epidermal growth factor receptor or HER2\/neu gene product","volume":"50","author":"Fendly BM","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_33_2","first-page":"588","article-title":"Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues","volume":"55","author":"Damjanov J","year":"1986","journal-title":"Lab Invest"},{"key":"e_1_2_1_34_2","first-page":"4726","article-title":"Expression of epidermal growth factor receptor in human cultured cells and tissues: Relationship to cell lineage and stage of differentiation","volume":"46","author":"Real FX","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12285389"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(87)90086-X"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711640403"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-11-1830"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb04016.x"},{"key":"e_1_2_1_40_2","first-page":"7106","article-title":"Suppression of an epidermal growth factor receptor hyperproducing tumor by an immunotoxin conjugate of gelonin and a monoclonal anti\u2010EGF R antibody","volume":"49","author":"Hirota N","year":"1989","journal-title":"Cancer Res"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240490208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240490208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T17:30:00Z","timestamp":1698168600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240490208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,6]]}},"alternative-id":["10.1002\/jcb.240490208"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240490208","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6]]}}}